栝楼籽多糖提取工艺优化及抗氧化性  

Optimization of ultrasonic-assisted extraction and antioxidant activity of polysaccharides from seed of Trichosanthes kirilowii Maxim

作　　者：熊利芝[1,2] 付柏婷 吴玉先[2] 王金理 何则强[2] 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [2]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《应用化工》2015年第6期1008-1011,共4页Applied Chemical Industry

基　　金：国家自然科学基金项目(51262008;51202087;51364009);湖南省自然科学基金项目(14JJ4048;12JJ2005);湖南省科技厅工业支撑计划重点项目(2012GK2017);湖南省高校创新平台开放基金项目(13K094)

摘　　要：采用超声波辅助法提取栝楼籽多糖。考察所得栝楼籽多糖对羟基自由基和O-2·自由基的清除能力。结果表明,超声波辅助法提取栝楼籽多糖的最佳提取工艺为:料液比1∶20(g/m L),在70℃条件下用175 W超声辅助提取60 min,栝楼籽中多糖提取率可达到3.60%左右。该多糖对·OH自由基清除能力强于柠檬酸和抗坏血酸,但其对O-2·自由基清除能力弱于抗坏血酸和柠檬酸。Polysaccharides from seed of Trichosanthes kirilowii Maxim was extracted by ultrasonic technology. Meanwhile,antioxidant activity of polysaccharides on·OH radical andO-2· radical was evaluated.Results showed the optimal ultrasonic-assisted extraction technology of polysaccharides in seed of Trichosanthes kirilowii Maxim is： material-to-liquid ratio of 1∶ 20（ g/m L）,ultrasonic power of 175 W,ultrasonicassisted extracting time about 60 min and extraction temperature at 70 ℃. The extraction rate of polysaccharides reaches about 3. 60% with the optimal ultrasonic-assisted extraction technology. The scavenge capability of ·OH radical is not only superior to citric acid,but to ascorbic acid,that ofO2^-· radical is inferior to citric acid and ascorbic acid.

关 键 词：栝楼 多糖 提取 抗氧化性 

分 类 号：TQ281.1[化学工程—有机化工]