基于混合分离分析策略发掘控制玉米分蘖性状位点  

Mining of Loci Controlling Tillering Characters in Maize via Bulked Segregation Analysis(BSA)

作　　者：王久光[1] 王瑷[1] 丁晓雨[1] 刘朝显[1] 刘志斋[1] 王国强[1] 蔡一林[1] 

机构地区：[1]西南大学农学与生物科技学院玉米研究所,重庆400715

出　　处：《西南大学学报（自然科学版）》2014年第10期42-48,共7页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市重大攻关项目资助(CSTC2012ggC80003;CSTC2012ggC80004;CSTC2012ggC80007);中央高校基本科研业务费专项基金资助(XDJK2011C005)

摘　　要：分蘖性状不仅与玉米的进化密切相关，对玉米育种也具有重要意义。利用具有共同系谱来源的8个分蘖自交系与3个不分蘖自交系，提取 DNA 并分别等量混合，形成分蘖与不分蘖基因池；采用混合分离分析策略（bulked segregation analysis ，BSA），利用覆盖玉米全基因组的1013个 SSR 标记对2个基因池进行多态性筛选，共获得了16个与玉米分蘖相关联的位点。这16个位点分布在玉米7条染色体上，其中包括2，4，5，6，7，8和9号染色体。参照IBM22008高密度整合遗传连锁图谱，这16个位点分别位于11个 Bin 区段中，基因功能注释显示这些片段中基因的数量在7～37个之间。这些研究结果为克隆控制玉米分蘖基因奠定了良好的基础。Tillering is of great importance in maize （Zea mays L. ） evolution and breeding .Genome DNA was extracted from 8 inbred lines with tillers and 3 with no tillers ,all derived from the same hybrid Chengdan 7 ,and was bulked with equal amount to form bulked gene pools by the tillering lines and non-tillering lines ,respectively .Through the polymorphism screening of 1 013 SSRs covering the entire maize genome ,16 loci related to the traits of tillering in maize were identified via bulked segregation analysis （BSA） between the two bulked gene pools .These 16 loci were found to be located on 7 chromosomes ,i.e. Chr. 2 ,Chr.4 ,Chr.5 ,Chr.6 ,Chr.7 ,Chr.8 ,and Chr.9 .Referring to the high density linkage map of IBM2 2008 Neighbors Frame ,these 16 loci were integrated into 11 separate chromosomal regions ,within which 7 to 37 genes were annotated .Hopefully ,these results may provide useful references for the resear-ches in the fields of gene cloning ,functional analysis and marker-assisted selection （MAS） .

关 键 词：玉米 分蘖 混合分离分析 基因发掘 

分 类 号：S513[农业科学—作物学]