siRNA沉默survivin增强hNIS转染的鼻咽癌细胞株对^(131)碘的敏感性  

Survivin special siRNA enhanced radiosensitivity of nasopharyngeal carcinoma cells transferred human sodium/iodide symporter gene to ^(131)I

作　　者：钟兴[1] 弓健[2] 郭斌[2] 徐浩[2] 

机构地区：[1]暨南大学附属第一医院医学影像中心超声科,广东广州510632 [2]暨南大学附属第一医院核医学科,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2015年第3期246-250,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省医学科研基金项目(A2012342);广东省科技计划基金项目(2011B061200028)

摘　　要：目的:研究Survivin特异性小片段干扰RNA(siRNA)能否增强人钠碘同向转运体(hNIS)转染的鼻咽癌细胞株(CNE-2-hNIS)对131碘的放射敏感性.方法:设计并合成针对survivin基因的特异性siRNA,利用脂质体法转染CNE-2-hNIS细胞,通过实时荧光定量聚合酶链式反应(RT-qPCR)和Westernblot方法检测细胞survivin基因沉默效果.CCK-8、克隆形成实验和Annexin VFITC/PI双染流式细胞仪检测131碘孵育后对细胞增殖和凋亡的影响.结果:SurvivinsiRNA转染CNE-2-hNIS细胞72h后,细胞的survivin基因mRNA和蛋白表达明显降低,Si-survivin组较SiRNA-NC组细胞增殖率降低,131碘孵育后,Si-survivin组较SiRNA-NC组细胞增殖率降低,凋亡率增高,差别有统计学意义(P<0.05).结论:Survivin特异性siRNA能显著沉默CNE-2-hNIS细胞的survivin基因的表达,增加CNE-2-hNIS对131碘的放射敏感性.Aim：To explore the effects of siRNA-mediated survivin on the enhancement radiosensitivity of Nasopharyngeal Carcinoma cell transferred Human Sodium /Iodide Symporter Gene （CNE-2-hNIS）to 131 I.Methods：The survivin siRNA was successfully constructed and was transiently transfected into NPC cell line CNE-2-hNIS by liposome-mediated.The expression of survivin was detected by RT-qPCR and western blot.The proliferation and apoptosis of CNE-2-hNIS after treatment with 131 I in vitro were detec-ted by CCK-8 cell proliferation,colony formation assay and Annexin V-FITC /PI double-labeled flow cy-tometry.Results：Significant down-regulation of the survivin protein expression was found after transfec-tion of the survivin-siRNA in CNE-2-hNIS cells.With 131 I treatment,the rate of proliferation in survivin-siRNA group was gradually lesser,whereas the rate of cell apoptosis was gradually increased than those of SiRNA-NC group.Conclusion：The survivin special siRNA silenced survivin gene of CNE-2-hNIS and en-hanced the radiosensitivity of CNE-2-hNIS to 131 I.

关 键 词：SURVIVIN RNA干扰 鼻咽癌 钠/碘同向转运体 碘放射性同位素 

分 类 号：R736.1[医药卫生—肿瘤]