重组人硫氧还蛋白对大鼠内毒素肺损伤保护作用  

Protective effect of recombinant human thioredoxin-1 on LPS-induced acute lung injury of neonatal rats

作　　者：陈红武[1] 赵海燕[1] 胡旭初[2] 

机构地区：[1]南方医科大学南方医院新生儿科,广东广州510515 [2]中山大学中山医学院寄生虫学教研室,广东广州510080

出　　处：《暨南大学学报（自然科学与医学版）》2015年第3期256-260,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省科技计划基金项目(2012B031800140)

摘　　要：目的:探讨重组人硫氧还蛋白1(rh Trx-1)对新生SD大鼠急性内毒素肺损伤的保护作用.方法:制备、鉴定rh Trx-1;将新生SD大鼠分为对照组、脂多糖(LPS)实验组和LPS+rh Trx-1干预组.腹腔注射LPS(质量分数为5 mg/kg),制备急性内毒素肺损伤模型,干预组于LPS注射前30 min腹腔注射rh Trx-1(质量分数为10 mg/kg),观察注射后24 h各组新生SD大鼠的一般情况以及死亡率;重复实验,每组分别于实验后的2、8 h随机处死大鼠,提取肺组织用于病理切片观察.结果:(1)成功制备rh Trx-1;(2)LPS组大鼠表现出活动少,反应差,精神萎靡,毛发无光泽,口周发绀等全身炎症反应症状,濒死状态时表现为毛色青灰,呼吸浅表;LPS+h Trx-1干预组动物症状明显减轻;阴性对照组、LPS实验组和LPS+rh Trx-1干预组大鼠24 h死亡率分别为0%,67%、17%(2=14.400,P=0.001);(3)LPS组肺组织损伤严重,可见肺组织结构不完整,肺泡间隔明显增宽,肺泡腔内有红细胞、炎症细胞及浆液渗出,血管周围组织可见白细胞浸润,毛细血管有明显充血及扩张表现,随时间延长有加重趋势,LPS+rh Trx-1干预组病理改变较内毒素组明显减轻,肺泡结构尚正常,轻度肺水肿,肺泡间隔稍增宽,红细胞渗出明显减少,肺泡及肺间质中白细胞浸润减少.结论:重组人硫氧还蛋白1对急性内毒素肺损伤的新生大鼠具有保护作用.Aim：To study the protective effects of recombinant human thioredoxin-1 on LPS-induced acute lung injury of neonatal rats.Methods：Prepare recombinant human thioredoxin-1 and then prepare animal model of LPS-induced acute lung injury of newborn rats.The full-term newborn Sprague-Dawley rats aged seven days were randomly divided into three groups-negative control group,LPS group and rhTrx-1 intervention group.The rats of rhTrx-1 group were received intraperitoneal injection with rhTrx-1 10 milligram per kilogram,and then with LPS 5 milligram per kilogram in the same way half an hour lat-er,while LPS group were received intraperitoneal injection with LPS 5 milligram per kilogram,negative control group with 0.9% saline 0.1 ml.Their general state and mortality of 24 hour were observed.The lung morphology of 2 hour and 8 hour was observed by hematoxylin-eosin stain.Results：1.The prepara- 〈br〉 tion of rhTrx-1 was successful.2.In the negative control group,everything was normal,no deaths oc-curred.In the LPS group,rats gradually showed a decrease in activity,drowsiness,perioral cyanosis, shortness of breath and some systemic inflammatory response symptoms after LPS injected.It turned grey in the skin and they had shallow breathing when they were moribund.The mortality was 67%.In rhTrx-1 group,the general condition of rats were obviously better than that of the control group.The mortality was 17%.The differences among the three groups had statistical significance.3.In the negative control group,the structure of the lung tissue was complete and there was no edema in alveolar septum.In LPS group,the structure of the lung tissue was incomplete and alveolar septum widened.There were a large number of red blood cells,inflammatory cells and serous exudates in the alveolar.Leukocyte infiltration, capillary hyperemia and expansion was visible in perivascular tissue.Compared with LPS group,patho-logical change was significantly reduced in the rhTrx-1 group.The edema was mild,alveolar septum wid-ened slightly.Also,the leak

关 键 词：重组人硫氧还蛋白1 急性肺损伤 新生SD大鼠 

分 类 号：R365.563[医药卫生—病理学]