金钗石斛水溶性多糖分子量分布及单糖组份分析（英文）  

Molecular weight distribution and monosaccharide composition analysis for water-soluble polysaccharide from Dendrobium nobile Lindl

作　　者：姚晓东[1,2] 刘章泉 汪巍[1] 周旭美[1,2] 

机构地区：[1]遵义医学院药学院,贵州遵义563099 [2]遵义医学院院士工作站糖化学与糖生物学实验室,贵州遵义563099

出　　处：《遵义医学院学报》2015年第3期239-243,共5页Journal of Zunyi Medical University

基　　金：贵州省遵义医学院院士工作站建设项目(NO:C-768);教育部春晖计划项目(NO:B-035);遵义医学院招标课题(NO:F-681);遵义医学院硕士启动基金资助项目(NO:F-714)

摘　　要：目的考察金钗石斛水溶性多糖分子量分布并分析其单糖组成。方法金钗石斛水溶性粗多糖采用水提醇沉法获得,适量双蒸水复溶,再次醇沉,然后采用Savag法除去蛋白得到精制多糖。金钗石斛精制多糖的分子量分布采用高效凝胶渗透色谱(HPGPC)测得;多糖经水解、还原、乙酰化后采用气相色谱(GC)确定单糖组成。7种标准单糖(包括1种酮糖)同法处理。结果精制金钗石斛多糖主要由5部分组成,其保留时间分别为9.269、9.564、14.628、15.410和15.840 min,由面积归一化法计算各组分的百分含量依次是26.65%、32.36%、8.98%、20.70%和11.31%,其分子量则为9.2×104、8.1×104、4.5×102、1.2×102、6.1×10 Da。乙酰化标准单糖实现基线分离,精制金钗石斛多糖由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖6种酮糖组成,其摩尔比是:1.00:1.65:2.58:1.08:1.83:1.15。结论金钗石斛多糖主要由多糖及寡聚糖组成,其单糖组成为6种酮糖。Objective To explore the molecular weight distribution and analyze monosaccharide composition of water-soluble polysaccharide of Dendrobium nobile Lindl.（ws DNP）.Methods Crude ws DNP was obtained by hot-water extraction and high concentration ethanol precipitation.Purified ws DNP was acquired using water dissolving and ethanol precipitating,then the Savag method was used for deproteinization.The molecular weight distribution of purified ws DNP was carried out on a High Performance Gel Permeation Chromatography（HPGPC）,then subjected to hydrolysis,reduction,and acetylate,and injected into a Gas Chromatography（GC）.7 standard monosaccharides（include one ketose） were treated the same procedure as above mentioned.Results The purified ws DNP included five portion,whose retain time were 9.269,9.564,14.628,15.410 and 15.840 min,and their content ratio were 26.65%,32.36%,8.98%,20.70% and 11.31% according to Area Normalization Method.The estimated molecular weights were 9.2 × 10^4,8.1 × 10^4,4.5 × 10^2,1.2 × 10^2,and 6.1 × 10 Da,respectively.Acetylated standard monosaccharides were separated on baseline.The purified ws DNP included 6 aldose,namely,rhamnose,arabinose,xylose,mannose,glucose and galactose,and its molar ratio was 1.00：1.65：2.58：1.08：1.83：1.15.Conclusion The ws DNP consists polysaccharide and oligosaccharide,and its monosaccharide composition was 6 aldose.

关 键 词：分子量分布 HPGPC 单糖组成 GC 多糖 金钗石斛 

分 类 号：Q539[生物学—生物化学]