基于杨木纤维发酵产丁醇工艺条件的研究  

Study on fermentation condition for producing butanol from poplar fibers

作　　者：钟洁[1] 王义强[1,2,3] 华涟滩 王启业[1] 罗浪[1] 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙410004 [2]中南林业科技大学生物技术实验室,湖南长沙410004 [3]中南林业科技大学国家林业局生物乙醇研究中心,湖南长沙410004

出　　处：《中南林业科技大学学报》2015年第7期125-130,共6页Journal of Central South University of Forestry & Technology

基　　金：国家林业局"948"项目(2011-4-13)

摘　　要：丁醇作为新一代生物燃料,已经成为世界研究的热点。利用本实验选育的丁醇高产突变株——拜氏梭菌Clostridium beijerinckii U-57,以杨木蒸汽爆破渣为原料发酵产丁醇。结果表明:杨木蒸汽爆破渣糖化液经分步糖化发酵和同步糖化发酵,丁醇产量分别为2.19、1.79 g/L;进而对同步糖化发酵条件进行正交设计优化,优化后同步糖化发酵得到的丁醇和总溶剂产量分别为2.16、3.44 g/L,比之前提高了20.7%、16.7%。该研究首次探讨了杨木纤维发酵产丁醇的工艺条件,为进一步提高产量提供了基础。Butanol, as a new generation ofbiofuel, has become one of the research hotspots in the world. The high-yield mutant strains （Clostridium beijerinckii U-57）, was bred by author in the early days of the study, and by taking the aspen steam explosion residues as the raw materials, the butanol was produced with fermentation process. The results show that the aspen steam explosion residue saccharificafion liquid were treated through separate hydrolysis fermentation and simultaneous saccharification fermentation （SSF）, and butanol was obtained, the yield of butanol were 2.19 g/L and 1.79 g/L, respectively; By optimizing the conditions of SSF, the production of butanol and total solvent were 2.16 g/L and 3.44 g/L, which were 20.7 % and 16.7 % higher than before. This study firstly discussed the fermentation condition of poplar fiber to produce butanol, which provides some foundations to enhance the yield of butanol.

关 键 词：生物燃料 丁醇 杨木纤维 拜氏梭菌U-57 同步糖化发酵 

分 类 号：S792.11[农业科学—林木遗传育种]