微RNA-429调控人乳腺癌Bcap37细胞系的增殖和迁移能力  

miRNA-429 regulates the proliferation and migration of human breast cancer cell line Bcap37

作　　者：秦科宇 李恒宇[1] 许鹿[1] 盛湲[1] 

机构地区：[1]第二军医大学附属长海医院甲乳外科,上海200433

出　　处：《中华乳腺病杂志（电子版）》2015年第2期16-20,共5页Chinese Journal of Breast Disease(Electronic Edition)

基　　金：长海医院"1255"特色学科培育基金(CH125530701)

摘　　要：目的通过在人乳腺癌Bcap37细胞系中过表达miRNA-429,检测miRNA-429对乳腺癌细胞增殖和迁移能力的影响。方法用miRNA-429过表达慢病毒及无miRNA-429过表达作用的空白慢病毒分别感染人乳腺癌Bcap37细胞后,建立稳定转染细胞株作为实验组和对照组,利用实时PCR探针法检测两组miRNA-429的表达水平;运用MTT法检测miRNA-429对乳腺癌Bcap37细胞增殖的影响;利用划痕实验及Transwell细胞迁移实验检测miRNA-429对人乳腺癌Bcap37细胞迁移能力的影响。两组以上的数据采用单因素方差分析,组间两两比较采用LSD-t检验;两组数据采用t检验;具有多个时间点的组间比较采用重复测量资料的方差分析。结果感染miRNA-429过表达慢病毒后,实验组细胞株中的miRNA-429表达水平较之于对照组细胞株明显上调(2.88±0.02比1.00±0.05,t=10.33,P=0.009);过表达miRNA-429后,实验组细胞增殖能力较之于对照组增强(t=11.95,P=0.000),划痕实验和Transwell实验均显示实验组细胞的迁移能力较之于对照组明显增强[(605±23)个比(365±21)个,t=13.30,P=0.001]。结论过表达miRNA-429可显著增加人乳腺癌Bcap37细胞的增殖和迁移能力。Objective To investigate the effect of miRNA-429 on proliferation and migration of human breast cancer Bcap37 cells after overexpressing miRNA-429. Methods The miRNA-429-overexpressed lentivirus and the blank lentivirus were infected into human breast cancer Bcap37 cells to establish stably transfeeted cells as the experimental group and the control group respectively. Real-time PCR was used to detect the expression level of miRNA-429 after infection; MTT assay was used to detect the effect of miRNA-429 on cell proliferation of Bcap37 ; the scratch test and TransweU experiment were used to detect the effect of miRNA-429 on migration ability of Bcap37 cells. The data from over 2 groups were analyzed by one-way variance analysis and pairwise comparison was conducted by ISD-t test. The data between two groups were compared by t test. The data from multiple time points were compared by analysis of variance of repeated measurement. Results After infecting the miRNA-429-overexpressed lentivirus, the expression level of miRNA-429 in Bcap37 cells of experimental group was significantly up-regulated compared with the control group （2. 88±0.02 vs 1. 00±0.05,t = 10. 33 ,P=0. 009）. The proliferation ability of Bcap37 cells in experimental group was enhanced compared with the control group （F= 189. 410 ,P=0. 000）. The scratch test and Transwell experiment showed that the migration ability of Bcap37 cells was increased after overexpression of miRNA-429 compared with the control group （605±23 vs 365±21, t = 13. 30, P = 0. 001 ）. Conclusion The overexpressed miRNA-429 can significantly increase the proliferation and migration abilities in human breast cancer cell line Bcap37.

关 键 词：乳腺肿瘤 微RNAS 细胞增殖 细胞运动 

分 类 号：R737.9[医药卫生—肿瘤]