microRNA-126靶向PIK3R2调控肝癌细胞SMMC-7721的增殖和凋亡  

Inhibitory effects of microRNA-126 on proliferation and apoptosis of human hepatocellular carcinoma cell line SMMC-7721 by targeting PIK3R2

作　　者：彭吉祥[1] 王小康[1] 林卓远 吴永定 叶剑恒 蔡志煅 杨盛帮[2] 江福能[2] 

机构地区：[1]广州市第一人民医院广州消化疾病中心,510180 [2]广州市第一人民医院中心实验室,510180

出　　处：《岭南现代临床外科》2015年第3期260-265,共6页Lingnan Modern Clinics in Surgery

基　　金：广州市科技计划项目(2013J4100025)

摘　　要：目的研究micro RNA-126(mi R-126)对肝癌细胞SMMC-7721迁移能力的影响以及对靶基因PIK3R2表达的影响。方法通过慢病毒转染肝癌细胞株SMMC-7721使其过表达mi R-126,利用CCK-8法检测细胞增殖能力。流式细胞术检测细胞凋亡;采用实时定量PCR和蛋白印迹检测细胞PIK3R2表达水平的变化,并通过荧光素酶实验验证mi R-126与PIK3R2基因的直接调控关系。结果与对照组相比,转染mi R-126组的SMMC-7721细胞的增殖能力减弱,凋亡增加。过表达mi R-126后,SMMC-7721细胞PIK3R2的蛋白表达下调(P=0.0135)。荧光素酶报告基因实验显示,mi R-126能明显抑制PIK3R2-3’UTR的荧光素酶活性(P=0.0016)。结论过表达mi R-126可能通过靶向降低PIK3R2基因的蛋白表达,抑制肝癌细胞的迁移。Objective To explore the influence of microRNA-126（miR-126） on proliferation and apoptosisof human hepatocellular carcinoma cell line SMMC-7721 and evaluate the correlation between miR-126 and PIK3R2. Methods After transfection of microRNA mimics into SMMC-7721 cells,cell proliferation and apoptosis were assessed by CCK-8 assay and cell apoptosis assay.Expression of PIK3R2 was evaluated by quantitative real-time polymerase chain reaction and western blotting. Luciferase reporter assay was used to confirm the relationship between miR-126 and PIK3R2.Results The outcomes by CCK-8 assay and apoptosis assay revealed that transfection of miR-126 suppressed cell proliferation, induced cell apoptosis of SMMC-7721 cells. Ectopic overexpression of miR-126 caused significant decrease of protein level of PIK3R2（P =0.0135）. In addition, luciferase assay showed that miR-126 significantly weakened the normalized PIK3R2-3 ＇ UTR luciferase activity（P=0.0016）. Conclusion Overexpressed miR-126 can inhibit proliferation and promote apoptosis of hepatocellular carcinoma cells by downregulating PIK3R2, thus indicating its tumor suppressive function in hepatocellular carcinoma.

关 键 词：微小RNA126 肝癌 细胞增殖 细胞凋亡 PIK3R2 

分 类 号：R735.7[医药卫生—肿瘤]