蛋白酪氨酸磷酸酶-3对结肠癌细胞表皮生长因子相关信号通路的调节  

Regulatory effects of PRL-3 on epidermal growth factor signaling pathways in colorectal cancer

作　　者：黄伟华[1] 来伟[2] 篮球生 张旸[2] 褚忠华[2] 

机构地区：[1]广东省梅州市五华县人民医院普外科,广东514400 [2]中山大学孙逸仙纪念医院胃肠外科,广东510120

出　　处：《岭南现代临床外科》2015年第3期348-350,共3页Lingnan Modern Clinics in Surgery

基　　金：广东省科技计划国际合作项目(2012B050600014);梅州市科技计划项目(2014B142)

摘　　要：目的观察蛋白酪氨酸磷酸酶-3(PRL-3)在结肠癌Lo Vo细胞中对表皮生长因子(EGFR)相关信号通路的调节作用。方法转染PRL-3的Lo Vo细胞(以下简称Lo Vo-P)及对照组Lo Vo细胞(以下简称Lo Vo-C)48 h后,通过Western blot检测Lo Vo细胞PRL-3蛋白表达,并检测EGFR及相关信号MEK-ERK通路蛋白p-MEK1/2,p-Erk1/2,p-Msk1/2的表达。结果Western-Blot检测发现高表达PRL-3的Lo Vo细胞能够激活EGFR磷酸化水平,蛋白灰度分析显示其表达增多72.3%(P<0.01)。并且转染PRL-3后Lo Vo细胞的MEK-ERK通道被激活。结论 PRL-3通过激活细胞表皮生长因子磷酸化参与表皮生长因子相关信号通路的调节。Objective To observe protein tyrosine phosphatase-3（PRL-3） related regulatory function on epidermal growth factor（EGFR） signaling pathway in colon cancer LoVo cells. Methods After PRL-3 was transfected to LoVo cells（hereinafter referred to as LoVo-P） for 48 h,phosphorylation of EGFR protein expression and phosphorylation of MEK1 / 2（p-MEK1 / 2）, Erk1 / 2（p-Erk1 / 2）, Msk1 / 2（p-Msk1 / 2） from MEK-ERK pathway were detected by Western blot assay.Results After transfected PRL-3, LoVo cells activated MEK-ERK channel. LoVo-C had an ability to activate phosphorylation of EGFR and Western-Blot showed an increase of 72.0% when compare with control group LoVo cells. High PRL-3 expression also induced to expression of p-MEK1 / 2, p-Erk1 /2, p-Msk1 / 2. Conclusion In colon cancer cells, PRL-3 involved EGFR signaling pathway regulation by activation of phosphorylation of EGFR.

关 键 词：PRL-3 Lo VO EGFR MEK-ERK 

分 类 号：R735.35[医药卫生—肿瘤] R656.9[医药卫生—临床医学]