甲状腺微小乳头状癌中Galectin-3、CK19和HBME-1的表达及其意义  

Expression and significance of Galectin-3,CK19 and HBME-1 in papillary thyroid microcarcinoma

作　　者：贺家勇 付娟娟[2] 杨晨晨[3] 

机构地区：[1]中国石油乌鲁木齐石油化工总厂职工医院外一科,乌鲁木齐830019 [2]新疆维吾尔自治区人民医院病理科,乌鲁木齐830000 [3]新疆医科大学,乌鲁木齐830011

出　　处：《新疆医科大学学报》2015年第7期850-854,共5页Journal of Xinjiang Medical University

基　　金：新疆重大疾病医学重点实验室--省部共建国家重点实验室培育基地开放课题(SKLIB-XJMDR-2014-Y3)

摘　　要：目的探讨甲状腺微小乳头状癌中Galectin-3、CK19和HBME-1的表达及其临床意义。方法采用免疫组化Envision法检测甲状腺微小乳头状癌及甲状腺良性病变伴乳头状增生中Galectin-3、CK19及HBME-1的表达,并分析Galectin-3、CK19及HBME-1与临床病理的关系。结果 CK19的表达定位于胞浆中,CK19甲状腺微小乳头状癌和甲状腺良性病变伴乳头状增生中的阳性表达率分别为92.5%(37/40)和10.0%(4/40),差异有统计学意义。有38例(95.0%)Galectin-3阳性表达,阳性部位定位于细胞膜和细胞质上,呈弥漫性阳性表达。34例(85.0%)样本HBME-1呈中度阳性及强阳性着色,2例(5.0%)呈弱阳性着色,主要在细胞膜及少部分胞质中表达,阳性表达率为90.0%。17例(42.5%)乳头状癌中同时出现CK19、HBME-1及Galectin-3强阳性表达,而良性病变患者未见同时出现CK19、HBME-1及Galectin-3的强阳性表达。甲状腺微小乳头状癌中Galectin-3、CK19及HBME-1的阳性表达率明显高于甲状腺良性病变伴乳头增生,差异有统计学意义。CK19的高表达与性别有关,HBME-1的阳性表达与年龄有关,差异具有统计学意义(P<0.05)。结论 CK19、HBME-1及Galectin-3在甲状腺微小癌中的表达明显高于甲状腺良性病变伴乳头状增生,是诊断甲状腺微小乳头状癌的重要标志物,联合检测更能提高甲状腺微小乳头状癌的准确性。Objective To investigate expression and clinical significance of Galectin-3,CK19 and HBME-1 in papillary thyroid microcarcinoma.Methods Envision immunohistochemistry was used to detect the ex-pression of Galectin-3,CK19 and HBME-1 in papillary thyroid microcarcinoma and benign thyroid lesions;and their clinical pathology relationship was analyzed.Results The positive expression rate of CK19 in pa-pillary thyroid microcarcinoma and benign thyroid disease was 92.5%（37/40）and 10.0%（4/40）respective-ly,and the difference was statistically significant.There were 37 cases （92.5%）showed positive staining of Galectin-3,three cases （7.5%）were weakly positive staining.The positive part was at the cell mem-brane and cytoplasm,diffuse positive expression.34（85.0%）cases showed positive staining of HBME-1＆amp;nbsp;and 2 cases （5.0%）were weakly positive staining.The positive expression rate was 90.0%.CK19,HBME-1 and Galectin-3 were all expressed in 17（42.5%）patients with papillary thyroid microcarcinoma,and no simultaneous expression of CK19,HBME-1 and Galectin-3 appeared in patients with benign lesions.The expression of Galectin-3,CK19 and HBME-1 in small papillary thyroid carcinoma was significantly higher than that in benign thyroid lesions with papillary hyperplasia,the difference was statistically significant. High expression of CK19 HBME-1 was related with gender,age,the difference was statistically significant （P〈0.05）.Conclusion The expression of CK19,HBME-1 and Galectin-3 in papillary thyroid microcarci-noma was significantly higher than that in benign lesions caused by papillary thyroid hyperplasia.The com-bined detection of CK19,HBME-1 and Galectin-3 will further improve the accucacy of papillary thyroid microcarcinoma.

关 键 词：甲状腺微小乳头状癌 GALECTIN-3 CK19 HBME-1 标志物 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]