BRMS1和Cx43蛋白异常表达与甲状腺癌发生发展关系的研究  

Abnormal expression of BRMS1 and Cx43 protein in thyroid cancer occurrence and progression

作　　者：吴宇[1] 刘辉[1] 

机构地区：[1]福建医科大学教学医院,福建省肿瘤医院头颈外科,福州市350014

出　　处：《中国肿瘤临床》2015年第11期550-554,共5页Chinese Journal of Clinical Oncology

基　　金：福建省卫生厅青年科研计划(编号:2012-2-14)资助~~

摘　　要：目的:探索甲状腺癌组织中乳腺癌转移抑制基因1(breast cancer metastasis suppressor 1,BRMSl)和细胞缝隙连接蛋白43(connexin 43,Cx43)的表达与患者临床资料、预后的关系。方法:收集2009年1月至2013年6月间福建省肿瘤医院头颈外科采用免疫组织化学SP法检测195例组织石蜡标本中BRMS1和Cx43蛋白表达情况,其中包括甲状腺癌90例,甲状腺腺瘤45例,结节性甲状腺肿和正常甲状腺组织各30例。结果:甲状腺癌组织中BRMS1和Cx43蛋白的阳性表达率分别为56.7%(51/90)和41.1%(37/90),均显著低于其他3组组织标本(均P<0.001)。Cx43蛋白在不同病理类型组织中阳性表达率差异有统计学意义(均P<0.05):乳头状癌61.9%,髓样癌27.8%,滤泡状癌27.3%,未分化癌12.5%;而BRMS1的表达未见显著性差异。BRMS1和Cx43蛋白在淋巴结转移患者癌组织中表达率均显著低于无淋巴结转移患者(均P<0.001);亚组分析显示随着肿瘤TNM分期的改变,BRMS1和Cx43蛋白的阳性表达率逐渐下降,且两种蛋白表达呈正相关(r=0.494,P=0.032)。结论:BRMS1和Cx43蛋白在甲状腺癌组织中表达均显著下降,且其表达差异与甲状腺癌的浸润转移、肿瘤恶性程度及TNM分期有关,二者联合检测或有利于复发转移的判断。Objective： To investigate the protein expression levels of breast cancer metastasis suppressor 1 （BRMS1） and Con- nexin 43 （Cx43） in thyroid cancer and their correlation with clinicopathologic parameters. Methods： Immunohistochemistry Streptavi- din-Peroxidase method was used to detect the BRMS 1 and Cx43 protein expression in 195 tissue samples, including 90 cases with thy- roid carcinoma, 45 cases with thyroid adenoma, 30 cases with nodular goiter, and 30 cases with normal thyroid glands. Results： The positive rates of BRMS1 and Cx43 expression was 56.7% （51/90） and 41.1% （37/90） in thyroid carcinoma, respectively, which are sig- nificantly lower than the rates in thyroid adenoma, nodular goiter, and normal thyroid gland tissues （P〈0.001）. Of the three pathological types of thyroid cancer, the positive expression rate of Cx43 was 61.9% in papillary carcinoma, 27.8% in medullary carcinoma, 27.3% in follicular carcinoma, and 12.5% in undifferentiated carcinoma. Statistical differences in the BRMS 1 expression among papillary car- cinoma and the other pathological types were also noted. Unlike the patients without lymph node metastasis, the positive expression of BRMS1 and Cx43 proteins were both significantly low among patients suffering from nodal metastasis. Subgroup analysis shows that the positive expression of BRMS1 and Cx43 protein gradually decreased with TNM staging. In addition, a positive correlation was ob- served between the BRMS1 and Cx43 protein expression （r=0.494, P=0.032）. Conclusion： The decreased expression of BRMS1 and Cx43 proteins is significantly correlated with the metastasis of thyroid cancer and malignant grade. The combined detection of the two proteins can be ideal biomarkers for judging the prognosis of thyroid carcinoma.

关 键 词：甲状腺癌 乳腺癌转移抑制基因1 连接蛋白43 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]