实验性自身免疫性脑脊髓炎鼠模型建立及其病理特点  

Mouse Models and the Pathological Characterization of Experimental Autoimmune Encephalomyelitis Induced by MOG_(35-55)

作　　者：张健[1,2,3] 曾育琦[1,2,3] 张静[2,3,4] 康德勇[5] 黄天文[1,2,3] 陈晓春[1,3,4] 

机构地区：[1]福建医科大学附属协和医院神经内科,福州350001 [2]福建医科大学附属协和医院老年病科,福州350001 [3]福建省老年医学研究所,福州350001 [4]福建省高校老化与变性病重点实验室,福州350001 [5]福建医科大学附属协和医院病理科,福州350001

出　　处：《福建医科大学学报》2015年第1期16-19,共4页Journal of Fujian Medical University

基　　金：福建省临床重点专科建设项目

摘　　要：目的建立髓鞘少突胶质细胞糖蛋白多肽（MOG35-55）诱发的实验性自身免疫性脑脊髓炎（EAE）小鼠模型,并观察其病理特点。方法应用MOG35-55多肽加福氏完全佐剂皮下注射免疫雌性C56BL/6小鼠,观察其临床症状、病理改变及影像学变化。结果模型组小鼠发病时间为免疫后（12±4）d（8-16d）,发病率83.3%,呈慢性单向过程;H-E染色模型鼠脊髓白质见大量炎症细胞浸润;罗克沙尔坚牢蓝染色显示,脊髓白质呈片状髓鞘脱失;电镜显示,髓鞘内层呈板层剥脱,轴索肿胀,结构疏松;脊髓MRI检查可见髓内斑片状T2异常高信号。结论慢性EAE模型具有发病率高、死亡率低、模型稳定、重复性高、制作方便的特点,模型病理改变接近多发性硬化（MS）,是研究MS较为理想的动物模型。Objective To establish mouse models of experimental autoimmune encephalomyelitis（EAE）induced by peptide myelin oligodendrocyte glycoprotein（MOG35-55）and study their pathological characterization. Methods The female EAE model of C57BL/6 mice（10-12weeks）were immunized subcutaneously at four sites into the flanks with 300μg of myelin oligodendrocyte glycoprotein peptide（MOG35-55）with the assistance of Complete Freund＇s Adjuvant（CFA）and Pertussis toxin（PTX）. We observed the clinical symptoms,histopathologic changes and changes on magnetic resonance scan.Results The experimental group developed the typical symptoms of EAE on（12±4）days after immunization with the incidence of 83.3% and showed a chronic monophasic course. There was a large number of inflammatory cells infiltration and demylination inthe lumbar spinal cord. Electron micrographs demonstrated a considerable amount of the myelin sheaths displayed loose,vacuoles and splitting. Intramedullary spinal MRI study showed patchy T2 hyperintensityin EAE mice. Conclusion Our study reports a MOG-induced EAE model that has a high incidence and its pathologic changes were similar to multiple sclerosis（MS）,so it might be an ideal model for the research on MS.

关 键 词：少突神经胶质/免疫学 髓鞘 糖蛋白类/免疫学 脑脊髓炎 自身免疫性 实验性 模型 动物 

分 类 号：R744.5[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]