Sema4C在喉鳞状细胞癌组织中的表达及意义  

Expression and significance of Sema4C in laryngeal squamous carcinoma

作　　者：秦杰升[1] 洪良利[2] 林旭妮[1] 王挥戈[1] 邱前程[2] 

机构地区：[1]汕头大学医学院第一附属医院耳鼻喉头颈外科,广东汕头515041 [2]汕头大学医学院第一附属医院病理科,广东汕头515041

出　　处：《癌变．畸变．突变》2015年第3期230-233,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：汕头市2012年医疗重点科技计划项目

摘　　要：目的:探讨脑信号蛋白4C(Sema4C)在喉鳞状细胞癌组织中的表达及意义。方法:采用免疫组化En Vision二步法检测82例喉鳞状细胞癌及癌旁正常喉黏膜组织中Sema4C和微小染色体维持蛋白2(MCM2)蛋白的表达水平,用D2-40标记淋巴管,计数微淋巴管密度(LMVD),分析三者的表达水平变化与临床病理指标的关系。结果:Sema4C、MCM2蛋白在喉鳞状细胞癌组织中的阳性率分别为60%(49/82)及98%(80/82),均显著高于癌旁正常喉黏膜组织(P<0.01),LMVD在癌及癌旁喉黏膜中的差别无统计学意义(P=0.077);Sema4C、MCM2的表达水平与颈部淋巴结转移有关,与年龄及肿瘤T分级无显著相关。Sema4C、MCM2蛋白表达水平和LMVD,两两间均呈正相关关系(P<均0.05)。结论:Sema4C的异常高表达与喉鳞状细胞癌的发生发展、转移及预后有关,提示Sema4C有可能成为判断喉鳞状细胞癌生物学行为及预后指标。OBJECTIVE： To investigate the expression of semaphorin 4C（Sema4C） in laryngeal squamous carcinoma and its role in tumor proliferation and lymphatic metastasis. METHODS：We investigated the expression of Sema4 C and MCM2 in 82 cases of laryngeal squamous carcinoma and normal laryngeal tissues by immunohistochemical technique.Furthermore,we used D2-40 to label the lymphatic microvessel and measure the lymphatic microvessel density（LMVD） to analyze their roles in laryngeal squamous carcinoma. RESULTS：The expressions of Sema4 C and MCM2 in laryngeal squamous carcinoma were significantly higher than that in the normal laryngeal tissues（P〈0.01）,but the difference of LVMD was not significant（P=0.077）. The aberrant expression rates of Sema4 C,MCM2 and LVMD in laryngeal squamous carcinoma were closely related to lymph node metastasis but did not correlate with age and T stage. LMVD in laryngeal squamous carcinoma should positive correlation with the expressions of Sema4C（P〈0.05） and MCM2（P〈0.01）. Moreover,there was positive correlation between Sema4 C and MCM2（P〈0.05）.CONCLUSION：Sema4C may play a role in the development of laryngeal squamous carcinoma. It may be possible to follow the progression of the disease and provide prognosis by detecting the expression of Sema4 c.

关 键 词：喉鳞状细胞癌 脑信号蛋白4C 微小染色体维持蛋白2 免疫组化 微淋巴管密度 

分 类 号：R730.2[医药卫生—肿瘤]