青蒿琥酯增强顺铂对人肝癌HepG2细胞的细胞毒作用  

Artesunate enhanced the cytotoxic effect of cis-platinum on human HepG2 cells

作　　者：陈淑怡[1] 姚朗[1] 姜浩[1] 张文娟[1] 刘云岗[1] 李荣[2] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515 [2]南方医科大学附属南方医院肿瘤科,广东广州510515

出　　处：《热带医学杂志》2015年第5期580-582,共3页Journal of Tropical Medicine

基　　金：国家自然科学基金(81001259);广东省科技计划项目(2012B031500009)

摘　　要：目的研究青蒿琥酯（Art）及其联合顺铂（DDP）对人肝癌Hep G2细胞增殖和凋亡的影响。方法分别采用不同浓度的Art（0.16、0.8、4、20、100μmol/L）、DDP（0.16、0.8、4、20、100 mg/L）及Art（4μmol/L）和DDP（4 mg/L）联合处理Hep G2细胞24 h、48 h和72 h,MTT实验检测细胞增殖情况,流式细胞术检测Art（4μmol/L）组、DDP（4mg/L）组和Art（4μmol/L）＋DDP（4 mg/L）联合处理组细胞48 h的凋亡状况。结果随着处理时间延长（24~72h）,Art和DDP对Hep G2细胞生长的抑制/杀伤作用明显增强;它们在暴露72 h的条件下分别在0.16μmol/L和0.8 mg/L以上浓度具有浓度依赖性细胞毒作用。Art联合DDP处理细胞组对人肝癌Hep G2细胞的增殖抑制作用比单用Art或DDP组更强（P〈0.05）;人肝癌Hep G2细胞48 h的早期凋亡率分别为：Art组23.5%,DDP组27.8%,Art＋DDP联合用药组49.7%,差异有统计学意义（P〈0.05）。结论青蒿琥酯可抑制人肝癌Hep G2细胞增殖并诱导其凋亡;它与顺铂联合应用可能增强后者对人肝癌Hep G2细胞的细胞毒作用。Objective To explore the effect and mechanism of combined artesunate（Art）and cis-platinum（DDP）on cell proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2. Methods HepG2 cells were treated for24 hours, 48 hours and 72 hours with different concentrations of Art（0.16, 0.8, 4, 20 or 100 μmol / L）, DDP（0.16,0.8, 4, 20 or 100 mg / L）, or both Art（4 μmol / L） and DDP（4 mg / L）. The cell viability of HepG2 was detected by MTT assay. The cell apoptosis of HepG2 treated with Art（4 μmol / L） or DDP（4 mg / L） or both Art（4 μmol / L） and DDP（4 mg / L） was detected at 48 hours with flow cytometry（FCM） assay. Results The cell proliferation of HepG2 was inhibited in a concentration-dependent and time-dependent manner by Art or DDP treatment. Compared with Art or DDP treatment alone, Art could significantly enhance the inhibiting effect of DDP on the cell proliferation of HepG2（P〈0.05）. It was shown by flow cytometry（FCM） assay that the apoptosis rate of HepG2 cells at 48 hours of 4 μmol / L ART, 4 mg / L DDP and both 4 μmol / L ART and 4 mg / L DDP were 23.51%, 27.77% and 49.66%, respectively. The combined ART and DDP treatment could significantly increase the induction of apoptosis of HepG2 compared with ART or DDP treatment alone（P 〈0.05）. Conclusion Artesunate can effectively inhibit the proliferation and induce the apoptosis of HepG2 cells. Aresunate also can enhance the cytotoxic effect of cis-platinum on proliferation and apoptosis of HepG2 cells.

关 键 词：青蒿琥酯 顺铂 肝癌 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]