膀胱癌组织中DNMT蛋白表达水平与肿瘤增殖和凋亡程度的相关性  

Correlation of DNMT protein expression and tumor proliferation and apoptosis in the bladder carcinoma

作　　者：王全玉[1] 何炜[2] 蔺强[1] 李莉蕊[2] 吴振启[3] 

机构地区：[1]河北医科大学附属华北石油管理局总医院肿瘤科,河北任丘062550 [2]河北医科大学附属华北石油管理局总医院物理诊断科,河北任062550 [3]湖北医药学院,湖北十堰442000

出　　处：《现代肿瘤医学》2015年第13期1855-1858,共4页Journal of Modern Oncology

基　　金：湖北省卫生厅科研基金(编号:JX3B27)

摘　　要：目的:探讨膀胱癌组织中DNMT蛋白表达水平与肿瘤增殖和凋亡程度的相关性。方法:收集膀胱癌组织标本80例和正常膀胱组织标本80例进行DNMT蛋白免疫组化分析。同时选择ABS-1膀胱癌细胞分为空白组、对照组、实验组。空白组加入常规培养基,对照组转染50nmol/L的空载体,实验组转染50nmol/L DNMT1 siRNA载体,然后进行肿瘤的增殖与凋亡分析。收集细胞进行DNMT蛋白表达检测。结果:DNMT1蛋白在膀胱癌组织中的阳性表达率为42.5%,在正常膀胱组织中的阳性表达率为2.5%,对比差异明显(P<0.05)。转染后DNMT1在空白组、对照组、实验组中的相对表达量分别为0.512±0.014、0.485±0.037和0.100±0.034,对比差异明显(P<0.05)。实验组增殖性明显低于空白组和对照组,而细胞凋亡率明显高于空白组和对照组,组间对比差异均有统计学意义(P<0.05)。结论:膀胱癌组织与膀胱癌细胞中存在DNMT1蛋白过表达,抑制DNMT1蛋白表达能有效抑制肿瘤细胞的增殖和促进凋亡。Objective：To investigate the correlation of DNMT Protein exPression levels and tumor Proliferation and aPoPtosis in the bladder carcinoma. Methods：All 80 bladder tissue samPles and 80 normal bladder tissue samPles were collected for DNMT Protein immunohistochemical analysis. Selected ABS-1 bladder cancer cell line were divid-ed into the blank grouP,control grouP,exPerimental grouP,blank grouP were give the regular medium,control grouP were transfected with 50nmol/L of air carriers,exPerimental grouP were transfected with 50nmol/L DNMT1 siRNA vectors,then the tumor Proliferation and aPoPtosis were analyzed. Also collected cells for DNMT Protein detection. Re-sults：The DNMT1 Protein Positive exPression in bladder cancer tissues were 42. 5%,2. 5% in normal bladder tissue （P〈0. 05）. After transfection,the DNMT1 relative exPression amount in the blank grouP,the control grouP,the ex-PerimentalgrouPwere0.512±0.014,0.485±0.037and0.100±0.034（P〈0.05）.TheProliferativeratesinthe exPerimental grouP was significantly lower than the blank grouP and the control grouP,while the aPoPtosis rate was significantly higher than the blank grouP and the control grouP（P〈0. 05）. Conclusion：The DNMT1 Protein exPres-sion in the bladder cancer tissue and cells were higher,inhibition of DNMT1 Protein can inhibit tumor cell Prolifera-tion and induce aPoPtosis.

关 键 词：膀胱癌组织 DNMT蛋白 肿瘤增殖 肿瘤凋亡 

分 类 号：R737.14[医药卫生—肿瘤]