沉默MTA1表达对胃癌SGC7901细胞增殖和侵袭能力的影响  

Effect of MTA1 gene silencing by siRNA on proliferation and invasion of human gastric cancer cell SGC7901

作　　者：原甜甜[1] 衣艳梅[2] 李济伟[1] 林斌斌[1] 杨晓[1] 吴民华[2] 

机构地区：[1]广东医学院第一临床医学院,广东湛江524000 [2]广东医学院组织学与胚胎学教研室,广东湛江524000

出　　处：《现代肿瘤医学》2015年第14期1944-1947,共4页Journal of Modern Oncology

基　　金：国家自然科学基金项目(编号:81201763);国家及广东省大学生创新创业训练计划项目(编号:201310571005);广东医学院大学生创新实验项目(编号:ZZZF002);广东医学院科研项目(编号:M2012002)

摘　　要：目的:观察转移相关基因1(MTA1)基因沉默对人胃癌细胞SGC7901增殖和迁移侵袭能力的影响,探讨MTA1基因在胃癌发生发展中的作用。方法:利用脂质体法将靶向MTA1的小分子干扰RNA(siRNA)转染到胃癌细胞株SGC7901中。运用Real time PCR和Western blot技术检测SGC7901细胞内MTA1的mRNA和蛋白表达水平。CCK-8法检测细胞增殖能力,Transwell小室实验观察细胞迁移和侵袭能力。结果:MTA1 siRNA可有效抑制SGC7901细胞MTA1基因在mRNA和蛋白水平上的表达(P﹤0.01)。CCK-8实验表明MTA1 siRNA转染后细胞增殖不受影响;Transwell小室实验表明细胞迁移和侵袭能力明显下降。结论:沉默MTA1表达可抑制胃癌细胞株SGC7901迁移和侵袭,而不影响细胞增殖。MTA1在胃癌侵袭转移过程中可能发挥重要作用。Objeetive：To study the effect of silencing MTA1 gene expression on proliferation and invasion of human gastric cancer SGC7901 cell,and to explore the role of MTA1 in the development of human gastric cancer.Methods：Small interference RNA （siRNA） targeting MTA1 was transfected into SGC7901 cell by Lipofectamine 2000.The mRNA and protein expression level of MTA1 were detected by Real time PCR and Western blot.The cell proliferation was studied by CCK-8 assay.The cell migration and invasion ability was evaluated by Transwell assay.Results：Result of Real time PCR and Western blot indicated that siRNA could efficiently knock down expression of MTA1 （P ＜ 0.01）.CCK-8 result demonstrated that siRNA did not affect proliferation of SGC7901 cell（P ＞ 0.05）.The migration and invasion ability of SGC7901 cell was greatly impaired（P ＜ 0.01） by siRNA.Conclusion：The study provided evidence that MTA1 silencing inhibit migration and invasion ability of SGC7901 cell but had little effect on cell proliferation.MTA1 may play important role in invasion and metastasis of gastric cancer.

关 键 词：转移相关基因1(MTA1) 胃癌 SGC7901细胞 增殖 侵袭 

分 类 号：R730[医药卫生—肿瘤] R735.2[医药卫生—临床医学]