沉默Id1基因表达对卵巢癌细胞生长影响的实验研究  

The study of ovarian cancer of silence Id1 gene expression in vivo and vitro

作　　者：陈贝贝[1] 于丽波[1] 孙文洲[1] 戚伟华[1] 毕玉美[1] 刘澈[1] 尹洪丽[1] 陈鸿晶[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院妇科,黑龙江哈尔滨150081

出　　处：《现代肿瘤医学》2015年第14期1959-1962,共4页Journal of Modern Oncology

基　　金：黑龙江省自然科学基金(编号:D201047)

摘　　要：目的:通过构建人卵巢癌SKOV3 Id1特异性siRNA慢病毒载体及其裸鼠移植瘤模型,观察沉默分化抑制因子(inhibitor of differentiation-1,Id1)表达对卵巢癌SKOV3生长的影响。方法:根据Id1基因序列设计并合成的siRNA片段转染SKOV3细胞,将转染细胞分为四组:cell+lv-shRNA-Id1(实验组)、cell+lv-shRNA-NC(质粒对照组)、cell+lv-control(空病毒对照组)、cell(空白对照组),筛选出稳定转染的SKOV3细胞克隆,48小时后检测细胞生长活性。将40只裸鼠随机分为四组,分别接种上述转染成功的细胞,30天后称取瘤体重量,计算抑瘤率;然后通过Western blotting法检测移植瘤中Id1蛋白的表达。结果:在体外试验中,实验组的细胞生长活性显著低于空白对照组,具有统计学意义(P<0.05),三对照组间无明显差异(P>0.05);体内实验显示,实验组平均瘤重均低于其他三对照组,差异具有统计学意义(P<0.05),三对照组间瘤重差异无统计学意义(P>0.05);实验组、质粒对照组、空病毒组抑瘤率分别为45.98%、4.84%、4.50%;Western blotting结果显示,实验组中Id1表达较对照组明显下调(P<0.05)。结论:沉默Id1基因能抑制卵巢癌细胞的生长,有望成为卵巢癌治疗新靶点。Objective：To use nude mice SKOV3 ovarian cancer cell and xenografts,to observe the expression of Id1 （inhibitor of differentiation-1,Id1） on the growth of ovarian cancer SKOV3.Methods：According to Id1 gene sequence to design and synthesise siRNA fragments transfected SKOV3 cells,transfected cells were divided into four groups：Cell ＋ lv-shRNA-Id1 （experimental group）,cell ＋ lv-shRNA-NC （plasmid control group）,cell ＋ lv-control（empty virus control group）,cell （control group）.Cell growth activity were detected after 48 hours.The 40 mice were randomly divided into four groups,then inoculated successfully transfected cells described above.After 30 days,weight and calculate the tumor weight inhibitory rate.Then observe Id1 protein expression in tumor by Western blotting method.Results：In vitro,the growth activity of the cells in the experimental group was significantly lower than the control group（P ＜ 0.05）,no significant difference among the other three control groups（P ＞ 0.05）.In vivo experiments showed that the average tumor weight of the experimental group were lower than the other three control groups,the difference was statistically significant（P ＜ 0.05）.Among three control groups of tumor weight were not statistically significant （P ＞ 0.05）.The tumor inhibition rates of the experimental group,the plasmids control group and the empty virus groups were 45.98％,4.84％,4.50％.Western blotting results showed that Id1 expression in the experimental group was significantly lower than the three control groups （P ＜ 0.05）.Conclusion：Silence Id1 gene can inhibit the growth of ovarian cancer cells,which may be a target gene for the of ovarian cancer.

关 键 词：ID1 卵巢癌 SKOV3 裸鼠 

分 类 号：R737.31[医药卫生—肿瘤]