MiR-34b和miR-155在非小细胞肺癌中的表达及临床意义  

Expression and clinical significance of miR-34b and miR-155 in non-small cell lung cancer

作　　者：殷玉敬 高辉 郭佳 冯立文 郭瑞芳 

机构地区：[1]包头肿瘤医院病理科,内蒙古包头014030 [2]包头肿瘤医院肿瘤综合内科,内蒙古包头014030

出　　处：《现代肿瘤医学》2015年第14期1993-1996,共4页Journal of Modern Oncology

摘　　要：目的:检测肺癌组织及癌旁正常组织中miR-34b和miR-155的表达水平及其与肺癌临床病理特征之间的关系。方法:收集50例非小细胞肺癌患者的肺癌组织和癌旁正常肺组织标本,运用Real-time PCR检测miR-34b和miR-155在肺癌组织和癌旁正常肺组织中的表达情况,分析其表达与肺癌临床病理特征之间的关系。结果:Real-time PCR检测显示在50例非小细胞肺癌组织中miR-34b的表达显著低于癌旁正常肺组织(P=0.019),而miR-155的表达显著高于癌旁正常肺组织(P=0.000)。MiR-34b和miR-155的表达与淋巴结转移情况显著相关(P均<0.05,miR-34b呈负相关,miR-155呈正相关);与性别、年龄、病理类型及肿瘤分化程度无明显相关;miR-34b的表达与临床病理分期无显著相关,而miR-155表达与临床病理分期显著相关。结论:MiR-34b和miR-155的异常表达与非小细胞肺癌的发生发展相关,其可能成为肺癌的筛查、诊断及治疗的肿瘤标志物。Objective：To investigate the expression of miR-34b and miR-155 in lung cancer tissue and adjacent normal tissue,and its relationship with chmicopathological features of lung cancer.Methods：The specimens of lung tumor tissue and adjacent normal lung tissue of 50 cases of lung cancer received surgical treatment in our department were collected.The expressions of miR-34b and miR-155 was detected by Real-time PCR.The correlations between the expression of miR-34b and miR-155 and clinicopathologic features were analyzed.Results：The expression of miR-34b in lung cancer tissue was lower than adjacent normal tissue （P =0.019）,and the expression of miR-155 in lung cancer was higher than adjacent normal tissue（P =0.000）.Significant negative correlation were observed between miR-34b expression levels and lymph node metastasis（P ＜ 0.05）,and significant positive correlation was observed between miR-155 expression levels and lymph node metastasis（P ＜0.05）.It had no relationship with gender,age,pathological types and cell differentiation grade.Moreover,the expression of miR-155 was closely related to p-TNM stage,the expression of miR-34 b was not correlated to the type.Conclusion：The abnormal expression levels of miR-34b and miR-155 in NSCLC tissue indicates that it may play a role in the progression of NSCLC,and may be a useful molecular marker for screening,diagnosis and treatment of NSCLC.

关 键 词：miR-34b MIR-155 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]