远程缺血后适应对脑缺血再灌注大鼠血脑屏障的保护作用  

Protective Effects of Remote Ischemic Postconditioning on Blood Brain Barrier in Focal Cerebral Ischemia Reperfusion Rats

作　　者：杨世亮[1] 周晓明[2] 谭帅[1] 黄欣怡[1] 洪华[1] 

机构地区：[1]中山大学附属第一医院神经科,广东广州510080 [2]中山大学附属第六医院康复科,广东广州510655

出　　处：《中山大学学报（医学科学版）》2015年第3期371-376,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(S2012010008539)

摘　　要：【目的】研究在大鼠脑缺血再灌注(IR)损伤模型中远程缺血后适应对血脑屏障的作用及相关机制。【方法】将SD大鼠96只随机分为3组:假手术组、单纯缺血再灌注组和远程缺血后适应组。利用线栓法制作大鼠脑IR模型,缺血1.5 h后恢复再通,再灌注72 h后对各组大鼠进行神经功能评分,染色检测脑梗死体积、梗死侧大脑半球脑组织伊文思蓝(EB)渗出量,再对紧密连接相关蛋白Claudin-5和Occludin的表达水平进行分析。【结果】与假手术组相比,单纯缺血再灌注组神经功能评分(9.9±2.0)、脑梗死体积(58.8±1.4)及EB的渗出量(5.7±0.6)均增加(P<0.01),同时Claudin-5和Occludin表达下降(P<0.01);与单纯缺血再灌注组比较,远程缺血后适应组大鼠神经功能评分(7.5±1.7)降低(P<0.01),脑梗死体积(33±5)减少(P<0.01),EB渗出量(2.7±1.2)减少(P<0.01),相应地Claudin-5和Occludin表达增加(P<0.01)。【结论】远程缺血后适应可以减轻脑缺血再灌注损伤,维持血脑屏障稳定性,可能与增加Claudin-5、Occludin的表达有关。[Objective] To explore the protective effects of remote ischemic postconditioning to blood brain barrier （BBB） in cerebral ischemia reperfusion （IR） injury and potential mechanisms involved.[Methods] Ninety-six SD rats were divided into three groups randomly：sham operation group,ischemia-reperfusion （IR） group and remote ischemic postconditioning group （RIP）.Rats were exposed to 1.5h of middle cerebral artery occlusion and then 72 h of reperfusion.Rats in remote ischemic postconditioning group were given brief occlusion and release of femoral arteries.After 72 h of reperfusion,neurological function scores,infarct volumes of brains,EB leakage of ischemia cerebral hemispheres,and the expression of tight junction （TJ） associated protein Claudin-5 and Occludin were tested and analyzed.[Results] Compared to sham group,rats in IR group showed elevated neurological function scores （9.9 ± 2.0） （P＜ 0.01）,infarct sizes （58.8 ± 1.4） （P＜ 0.01）,increased EB leakage （5.7-± 0.6） （P ＜ 0.01）,accompanied with the expression of Claudin-5 and Occludin down-regulated （P ＜ 0.01） at 72h after reperfusion.Compared to IR group,rats in RIP group showed decreased neurological function scores （7.5 ± 1.7） （P＜ 0.01）,infarct sizes （33 ± 5） （P＜ 0.01） and EB leakage （2.7 ± 1.2） （P＜0.01）,accompanied with increased Claudin-5 and Occludin expression （P ＜ 0.01）.[Conclusions] Remote ischemic postconditioning can reduce cerebral IR injury,which may be associated with up-regulating the expression of TJ associated proteins to maintain the stability of BBB.

关 键 词：脑缺血再灌注 远程缺血后适应 血脑屏障 紧密连接 

分 类 号：R743[医药卫生—神经病学与精神病学]