AEG-1在口腔鳞状细胞癌中的表达及与预后的关系  

Expression of AEG-1 in oral squamous carcinoma and its correlation with prognosis

作　　者：王秀梅[1] 郭阳[1] 魏圣华 曹潇方[1] 谢晓华[1] 

机构地区：[1]哈尔滨医科大学附属第二医院口腔科,哈尔滨150086

出　　处：《临床肿瘤学杂志》2015年第5期422-426,共5页Chinese Clinical Oncology

基　　金：黑龙江省教育厅科学技术研究项目(12541499);黑龙江省领军人才梯队后备带头人资助项目

摘　　要：目的：探讨星形细胞上调基因-1（ AEG-1）在口腔鳞状细胞癌中的表达及其与临床病理特征和患者预后的关系。方法应用Western blotting检测AEG-1在口腔鳞癌细胞系（ CAL27、SCC4、Tca8113、Tb、Ts）及人永生化表皮细胞HaCaT中的表达；应用免疫组织化学方法检测AEG-1在87例口腔鳞癌和20例正常口腔黏膜组织中的表达，并分析AEG-1表达与口腔鳞癌临床病理特征的关系；采用Kaplan-Meier法进行生存分析，Cox风险比例回归模型分析影响口腔鳞癌患者预后的独立因素。结果 AEG-1在口腔鳞癌细胞系CAL27、SCC4、Ts及Tb中高表达；AEG-1在口腔鳞癌和正常口腔黏膜组织中的高表达率分别为51.7％（45／87）和10.0％（2／20），差异有统计学意义（ P＝0.001）。口腔鳞癌的AEG-1表达与分化程度、临床分期、T分期及淋巴结转移有关；生存分析显示，AEG-1低表达组的5年生存率为38-7％，高于高表达组的18-0％，差异有统计学意义（ P＝0.001）；单因素生存分析显示AEG-1、临床分期、T分期及淋巴结转移为影响口腔鳞癌预后的因素；Cox多因素分析显示AEG-1是影响口腔鳞癌患者预后的独立因素（ RR＝1.948，95％CI：1.013～3.744，P＝0.046）。结论 AEG-1过表达与口腔鳞癌的发生、发展密切相关，可作为判断口腔鳞癌恶性程度和不良预后的参考指标。Objective To detect the expression of astrocyte elevated gene-1 （ AEG-1） in oral squamous cell carcinoma （ OS-CC） , and to investigate the relationship between expression of AEG-1 in OSCC and clinicopathological characteristics, prognosis. Methods The expression of AEG-1 was measured by Western blotting in OSCC cells（ CAL27, SCC4, Tca8113, Tb, Ts） and HaCaT cell. At the same time, the expressions of AEG-1 of 87 patients with OSCC and 20 cases of normal oral mucosa were detected by immu-nohistochemistry. The relationship between the expression of AEG-1 in OSCC tissues and clinical pathological characteristics were ana-lyzed. The overall survival of OSCC patients was estimated using the Kaplan-Meier method. The factors affecting the prognosis of OSCC patients were analyzed by Cox proportional hazard regression model. Results AEG-1 was high expressed in OSCC cells （ CAL27, SCC4, Ts and Tb） . The high-level expression rates of AEG-1 was 51.7%（ 45/87） and 10.0%（ 2/20） , respectively in OSCC and nor-mal oral tissues, in which there existed difference statistically （P=0.001）.The expression of AEG-1 was closely correlated with differ-entiation, clinical stage, T stage and lymph node metastasis. Univariate analysis showed AEG-1, clinical stage, T stage and lymph node metastases were prognostic factor of OSCC. Multivariate analysis showed AEG-1 was independent prognostic factors in OSCC（ RR=1.948,95%CI：1.013-3.744,P=0.046） . Conclusion The overexpression of AEG-1 might play an important role in the occurrence and development of OSCC, and it can be used as a reference index of the malignant degree and poor prognosis of OSCC.

关 键 词：口腔鳞癌 星形细胞上调基因-1( AEG-1) 预后 

分 类 号：R739.8[医药卫生—肿瘤]