Caveolin-1mRNA及蛋白在食管鳞状细胞癌中的表达及其与Cyclin-D1、P16的相关性研究  

Caveolin-1mRNA and protein expression in esophageal carcinoma and the Relationship with Cyclin D1 and P16

作　　者：赵醒[1] 赵宇阳[1] 程玉[1] 薛晶[1] 张刚[2] 

机构地区：[1]承德医学院附属医院病理科,067000 [2]河北大学附属医院肿瘤外科

出　　处：《中华保健医学杂志》2015年第2期116-118,共3页Chinese Journal of Health Care and Medicine

基　　金：承德市科学发展研究计划项目(20122138);承德市科研基金资助项目(201310);保定市研究计划指导项目(ZD20140360)

摘　　要：目的研究Caveolin-1 m RNA及蛋白在食管癌及癌旁正常食管黏膜中的表达及其意义,进一步探讨Caveolin-1与食管癌组织中细胞周期素-D1(Cyclin-D1)、P16及临床病理学指标的相关性。方法收集食管鳞癌及对应癌旁组织标本90例,分别采用RT-PCR、免疫组化方法检测Caveolin-1 m RNA及蛋白的表达情况,进一步分析Caveolin-1与食管癌组织中Cyclin-D1、P16及临床病理学指标的相关性。结果 Caveolin-1 m RNA及蛋白在食管癌中的表达均高于食管癌旁组织中的表达(P<0.05),且Caveolin-1 m RNA的表达与Cyclin-D1和淋巴结转移有关。结论 Caveolin-1在食管癌中高表达,可能与食管鳞癌的发生、发展以及浸润转移有关。Objective To investigate the expression of Caveolin-1 in esophageal carcinoma and normal esophageal squamous cell and its clinical significance. To further discuss the correlation between Caveolin-1 and clinico-pathological features and Cyclin-D1,P16. Methods SP immunohistochemical and RT-PCR analysis were used to detect the expression of Caveolin-1m RNA and protein in 90 cases of esophageal carcinoma and normal esophageal squamous cell. And further discuss the correlation between Caveolin-1 and clinico-pathological features and Cyclin-D1,P16. Results The expression of Caveolin-1 in esophageal carcinoma was higher than in normal esophageal squamous cell（P〈0.05）. The expressions of Caveolin-1 had correlation with lymph node metastasis（P〈0.05）. Conclusion The expression of Caveolin-1 is high in esophageal carcinoma,may be related with esophageal cancer development and metastasis.

关 键 词：食管鳞癌 CAVEOLIN-1 CYCLIN-D1 P16 RT-PCR 免疫组化 

分 类 号：R735.1[医药卫生—肿瘤]