肝癌肿瘤微环境对血管内皮细胞增殖及血管生成能力的影响  

Influence of Tumor Microenvironment of Hepatocellular Carcinoma on the Proliferation of Vascular Endothelial Cells and Vascular Angiogenesis Ability

作　　者：夏庆[1] 尹红梅[2] 沈阳[1] 刘静霞[1] 闫志平[1] 洪锦勇 周法庭[1] 刘肖珩[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物医学工程实验室,成都610041 [2]四川大学华西药学院,成都610041

出　　处：《生物医学工程学杂志》2015年第3期612-617,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(11372203;11172189);教育部高等学校博士学科点专项科研基金资助项目(20120181120058);国家基础科学人才培养基金能力提高资助项目(J1103604)

摘　　要：研究间接共培养条件下肝癌细胞培养基对血管内皮细胞增殖及血管生成能力的影响,初步探讨肿瘤微环境下血管新生的分子机制。体外培养人脐静脉内皮细胞株EA.hy926,与肝癌细胞株HepG2条件培养基进行共培养;四甲基偶氮唑盐(MTT)法检测肝癌细胞条件培养基对血管内皮细胞增殖的影响;血管管腔形成实验检测血管内皮细胞的血管生成能力。Western blot测定肝癌细胞条件培养基对血管内皮EA.hy926细胞血管内皮生长因子(VEGF)及其受体Flk-1表达的影响。结果表明,EA.hy926细胞经HepG2条件培养基处理后,其增殖能力明显增加。接种于Matrigel胶的EA.hy926细胞经HepG2条件培养基刺激后,形成管腔数目增加,成血管能力明显增强;同时,其胞内VEGF及其受体Flk-1的表达呈时间依赖性上调。肿瘤微环境下肝癌细胞作用于血管内皮细胞,可促进血管内皮细胞的增殖并提高其血管生成能力。To study the potential molecular mechanism of tumor angiogenesis in its microenvironment,we investigated the effects of HepG2 conditioned medium on the proliferation of vascular endothelial cell and vascular angiogenesis in our laboratory.Human umbilical vein endothelial EA.hy926 cells were co-cultured with HepG2 conditioned medium in vitro.The proliferation and the tubulogenesis of EA.hy926 cells were detected by teramethylazo salt azole（MTT）and tube formation assay,respectively.The results showed that the survival rate of the EA.hy926 cells was significantly increased under the co-culture condition.HepG2 conditioned medium also enhanced the angiogenesis ability of EA.hy926 cells.In addition,the expressions of intracellular VEGF and extracellular VEGFR（Flk-1）were regulated upward in a time-dependent manner.In conclusion,the proliferation of vascular endothelial cells and Vascula angiogenesis were improved under the condition of indirect co-culture.

关 键 词：肝癌细胞 血管内皮细胞 细胞增殖 血管生成 

分 类 号：R735.7[医药卫生—肿瘤]