门脉注射聚氨酯-四氢呋喃溶液制备犬门脉高压症模型  

A Canine Portal Hypertension Model Induced by Intra-portal Administration of Polyurethane-Tetrahydrofuran Solutions

作　　者：严小鹏[1,2,3] 任冯刚[1,2,3] 马佳[4] 董鼎辉[1,2,3] 薛飞[1,2,3] 吕毅[1,2,3] 

机构地区：[1]西安交通大学第一附属医院肝胆外科,西安710061 [2]西安交通大学先进外科技术与工程研究所,西安710061 [3]陕西省再生医学与外科工程技术研究中心,西安710061 [4]陕西省人民医院肿瘤外科,西安710068

出　　处：《生物医学工程学杂志》2015年第3期645-649,共5页Journal of Biomedical Engineering

基　　金：卫生部部属(管)医院临床学科重点资助项目(2010105);中央高校基本科研业务费专项基金资助项目(XKJC2013003);国家自然科学基金科学仪器专项资助项目(81127005)

摘　　要：介绍一种利用聚氨酯高分子材料和有机溶剂四氢呋喃配制的溶液制备犬门脉高压症动物模型的方法,并评价成模效果。选用西安本地杂种犬12只,取上腹部小切口,经门静脉推注8%(W/V)的聚氨酯-四氢呋喃溶液建立门脉高压症模型。分别在造模前、造模中、造模后4、8、12周测量实验犬门静脉压力,以造模前门静脉压力作为正常参考值,评估模型建立效果。结果显示经门静脉注射聚氨酯-四氢呋喃溶液后门静脉压力可立即升至造模前的2.5倍,4周后门静脉压力维持在造模前的1.5倍以上。该法制备犬门脉高压症模型操作简单、死亡率低、门静脉压力维持稳定,尤其是腹腔粘连极轻,门静脉的结构和位置保持正常,非常适合外科手术治疗门脉高压症方面的研究。This study was to build a canine portal hypertension model by intra-portal administration of high polymer material polyurethane and organic solvent tetrahydrofuran mixed solutions in order to evaluate the effectiveness of the model.Twelve local crossbreed dogs were selected randomly,with intra-portal administration of 8%（weight/volume）polyurethane-tetrahydrofuran solutions through an incision in the upper abdomen to build the portal hypertension model.We measured the portal vein pressure before modeling,during modeling,and four-,eight-,and twelveweeks after modeling,respectively.Then we evaluated the effectiveness of the model comparing values of data with those data obtained before modeling started,which were regarded as the normal values.The results showed that the portal vein pressure rose by 2.5times after the solution administrated instantly as much as that before modeling,and maintained at 1.5times after 4weeks.This method presents an easy operation,low animal mortality and reliable model of portal hypertension.Its less abdominal adhesions and its ability in keeping normal anatomic structure specially make it suit for surgical research of portal hypertension.

关 键 词：门脉高压症 动物模型 聚氨酯 犬 

分 类 号：R575.2[医药卫生—消化系统] R-332[医药卫生—内科学]