CASK在癫痫大鼠模型中影响癫痫发作的机制研究  

Mechanism research of calcium/calmodulin-dependent serine protein kinase in epileptic seizure in rat models of epilepsy

作　　者：刘震[1] 周艳 杨艺[3] 党圆圆[3] 戴宜武[1,3] 

机构地区：[1]中国人民解放军第三军医大学研究生管理大队,重庆400038 [2]北京市通州区妇幼保健医院内科,101100 [3]北京军区总医院附属八一脑科医院,北京100700

出　　处：《中华神经医学杂志》2015年第6期591-596,共6页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金面上项目（81271391）

摘　　要：目的 探讨钙调蛋白依赖性丝氨酸蛋白激酶（CASK）在氯化锂-匹罗卡品癫痫大鼠模型中不同时间点的表达及其参与癫痫发作的可能机制. 方法 30只SD大鼠按照随机数字表法分为对照组（n=5）和实验组（n=25）,实验组在点燃后进一步按照随机数字表法分为1、3、7、14、30d5组（n=5）.通过免疫组化、免疫荧光和Western blotting实验分别检测CASK在模型鼠海马组织中的表达变化.另将21只成年健康SD大鼠按照随机数字表法分为3组（n=7）,分别为转染组、空病毒组、对照组,水合氯醛麻醉3组大鼠后分别注入等量的CASK-RNAi-LV、LV-scrRNAi和生理盐水,点燃1h内观察此3组大鼠行为学的改变,同时运用Western blotting检测转染组与对照组大鼠CASK复合物下游分子NMDAR2b在点燃后的表达. 结果 （1）免疫组化及荧光染色结果提示,CASK仅在神经元中表达,在神经胶质细胞中不表达;实验组CASK阳性细胞数明显多于对照组;且CASK在癫痫大鼠的海马组织中表达模式为1d降至最低,后逐渐增高,直到30 d.（2）转染模型相关实验结果提示,转染组（CASK表达被抑制）大鼠癫痫发作频率和发作级别（发作20 min后）均较空病毒组及对照组大鼠明显降低,差异有统计学意义（P＜0.05）.对照组大鼠NMDAR2b在点燃1d后开始增高,3d达到高峰,10d下降;而转染组大鼠NMDAR2b在点燃后各个时间点的表达水平较对照组大鼠均明显降低,差异有统计学意义（P＜0.05）. 结论 CASK在癫痫大鼠中的表达量明显增加,可能通过NMDAR2b途径影响癫痫的发生与发展.Objective To investigate the calcium/calmodulin-dependent serine protein kinase （CASK） expression in the hippocampus of epileptic rat models and its mechanism.Methods Thirty healthy adult male Sprague-Dawley rats were randomly divided into 6 groups （n=5）：control group and experimental groups of 1,3,7,14 and 30 d after kindling by lithium chloride-pilocarpine;CASK expressions in the hippocampus tissues were measured by immunohistochemistry,immunoflurescence and Western blotting.Another 21 healthy adult male Sprague-Dawley rats were divided into 3 groups （n=7）：transfected group,empty adenoviral group and control group,and after anesthesia with chloral hydrate,the same volume of CASK-RNAi-LV,LV-scrRNAi and normal saline was given,respectively;the behavior changes of rats in the three groups were observed within one h of kindling;the expression of N-methel-D-aspartate receptor subunit 2b （NMDAR2b,the downstream of CASK complex）,in the transfected group and control group was further examined by Western blotting.Results CASK expressed only in neurons not gliacyte;and the number of CASK positive cells in experimental groups was larger than that in the control group.CASK expression in the hippocampus reduced to minimum level one d after kindling,and maintained at increased levels until 30 days.Both seizure frequency and seizure grade of the epilepsy in the transfected group were significantly decreased as compared with those in the empty adenoviral group and control group （P〈0.05）.NMDAR2b expression in the hippocampus of control group increased at the first 24 h of kindling and maintained for 3 days,and then,it decreased 10 days after kindling;NMDAR2b expression in the hippocampus of transfected group was significantly reduced as compared with that in the control group at all time points of kindling （P〈0.05）.Conclusions The CASK expression of epilepsy rat models is increased,which influences the occurrence and development of epilepsy.CASK is linked to epilepsy via its action on synaptic NM

关 键 词：钙调蛋白依赖性丝氨酸蛋白激酶 NMDAR2b 癫痫 突触 慢病毒 

分 类 号：R742.1[医药卫生—神经病学与精神病学]