阿尔茨海默病患者血清脑源性神经营养因子水平的改变及意义  

Change and its significance of serum levels of brain-derived neurotrophic factor in patients with Alzheimer disease

作　　者：王珊[1] 郑为娜 褚红珊 生晓娜[1] 孙文蕾 王斌斌[1] 张国华[1] 

机构地区：[1]河北医科大学第二医院神经内科河北省神经病学重点实验室,石家庄050000

出　　处：《脑与神经疾病杂志》2015年第3期176-179,共4页Journal of Brain and Nervous Diseases

基　　金：河北省自然基金(C2011206110)

摘　　要：目的研究阿尔茨海默病(AD)患者血清脑源性神经营养因子(BDNF)水平的改变及意义。方法对40例AD患者(AD组)用酶联免疫吸附法检测血清BDNF水平、聚合酶链反应-限制性片段长度多态性对APOE基因进行分型及神经心理评估,并与遗忘型轻度认知障碍(a MCI组)患者和正常对照者(正常对照组)进行比较,分析AD患者血清BDNF水平及与临床指标的相关性。结果与正常对照组相比,AD和a MCI血清BDNF水平差异无统计学意义(F=1.21,P=0.33);a MCI、轻度AD、中重度AD组间BDNF水平差异无统计学意义(F=2.31,P=0.08);相关分析发现是否携带APOEε4等位基因和简易精神状态检查(MMSE)评分不能影响血清BDNF水平(P>0.05)。结论血清BDNF水平既不能作为AD早期诊断的外周标志物,也不能反映AD病情的严重程度。ObjectiveToinvestigatethechangesofbrain-derivedneurotrophicfactor（BDNF）from peripheral serum in patients with Alzheimer disease （ AD） and evaluate the significance of changes .Methods Forty AD patients （ including 26 mild AD and 14 moderate-severe AD,AD group） were recruited to measure the serum levels of BDNF using enzyme-linked immunosorbent assay （ ELISA） .APOE genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP ） .Cognitive functions were assessed by neuropsychological tests .The results were compared with those of amnestic mild cognitive impairment patients （ aMCI group） and normal controls （NC group）.The alteration of serum levels of BDNF and the relationship between BDNF and clinical variables were analyzed in AD patients .Rseults Compared with NC group ,no significant differences for the serum levels of BDNF were observed in AD and aMCI groups （ F=1.21,P=0.33） .the serum levels of BDNF also showed no significant difference among aMCI ,mild AD,moderate-severe AD groups （F=2.31,P=0.08）.Correlation analysis found APOEε4（ carrier or non-carrier） and mini mental state examination （ MMSE） score had no effects on BDNF levels （ P〈0.05） .Conclusion Serum levels of BDNF could not be as a candidate peripheral marker for early diagnosis of AD .Serum levels of BDNF could not reflect the severity of AD .

关 键 词：脑源性神经营养因子 阿尔茨海默病 认知障碍 外周标志物 

分 类 号：R749.1[医药卫生—神经病学与精神病学]