泊马度胺联合CAR-T细胞对多发性骨髓瘤细胞株RPMI8226、U266细胞的体外杀伤作用  

Cytotoxity of pomalidomide combined CAR-T cell for multiple myeloma cell RPMI8226 and U266

作　　者：王蕾[1] 张姝婷[1] 欧剑锋[1] 白海[1] 

机构地区：[1]兰州军区兰州总医院血液科,730050

出　　处：《中华血液学杂志》2015年第6期497-500,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（81372132）

摘　　要：目的 观察CD 138-CAR-T细胞对人多发性骨髓瘤（MM）细胞株RPMI8226和U266细胞的杀伤作用,探讨泊马度胺对CD138-CAR-T细胞及其杀伤作用的影响.方法 采用CFSE/7-AAD双标法检测CD138-CAR-T细胞及其联合泊马度胺对RPMI8226、U266细胞的杀伤活性.ELISA法检测CD 138-CAR-T细胞分泌IFN-γ的变化.结果 CD 138-CAR-T细胞作用18h后,对RPMI8226、U266细胞的杀伤活性分别为（55.2±3.9）％、（85.1±2.4）％,对照组分别为（7.0±1.5）％、（12.5±2.1）％,差异均有统计学意义（P值均＜0.01）;与CD138-CAR-T细胞组比较,CD 138-CAR-T细胞联合泊马度胺（2.5 μg/ml）作用18h后,对RPMI8226、U266细胞的杀伤活性差异无统计学意义（P值均＞0.05）.与CD138-CAR-T细胞组比较,CD138-CAR-T细胞和MM细胞共培养组IFN-γ分泌水平显著增高;与共培养组比较,加入泊马度胺后能显著促进IFN-γ的释放,差异均有统计学意义（P值均＜0.01）.结论 CD138-CAR-T细胞对MM细胞及耐药细胞株均有明显的杀伤作用;其与MM细胞共培养能促进后者IFN-y的分泌;泊马度胺能促进CD138-CAR-T细胞分泌IFN-γ.Objectve To observe the cytotoxity of CD138-CAR-T cells on human multiple myeloma cell RPMI8226 and U266 ceils and explore the impact of pomalidomide on the cytotoxity of CD138-CAR-T on RPMI8226 and U266 cells.Methods The cytotoxity of CD138-CAR-T and CD138-CAR-T combined pomalidomide on RPMI8226 and U266 was detected by CFSE/7AAD.The effctor cells were co-cultured with target cells at 5：1 for 18 h,and then the supematant were collected and used for ELISA assays.Results After 18 h co-culture,the cytotoxity of CD138-CAR-T on RPMI8226 and U266 was significantiy higher than control （P＜0.01）.There was no significant change on the cytotoxity of pomalidoide combined with CD138-CAR-T on RPMI8226 and U266.The results showed that co-cultured system contribted to a markedly increased production of IFN-γ,after adding pomalidomide to the cocultured system.It can significantly enhance the production of IFN-γ,compared with CD 138-CAR-T alone.Conclusions CD138-CAR-T had significantly cytotoxity on U266 and RPMI8226.Pomalidomide could promote CD 138-CAR-T cells IFN-γ production.

关 键 词：多发性骨髓瘤 泊马度胺 T淋巴细胞 嵌合抗原受体 

分 类 号：R733.3[医药卫生—肿瘤]