牙本质支架促进人牙髓干细胞体外增殖与分化能力的研究  

Dentin scaffold promotes the proliferation and differentiation of human dental pulp stem cells in vitro

作　　者：杨雪超[1] 李晓宁 王伟东[1] 吕孝帅 

机构地区：[1]广州医科大学附属口腔医院.广州口腔疾病研究所.口腔医学重点实验室,广东广州510140 [2]烟台市口腔医院牙体牙髓科,山东烟台264000

出　　处：《牙体牙髓牙周病学杂志》2015年第6期342-347,共6页Chinese Journal of Conservative Dentistry

基　　金：广东省教育厅特色创新项目(2014TSCX103);广东省科技计划项目(2013-53);广东省医学科研基金项目(A2014328);广州市荔湾区科技计划项目(20131215074-1)

摘　　要：目的 :比较不同根管冲洗剂处理根管壁后对牙髓干细胞(h DPSCs)增殖及分化能力的影响。方法:用镍钛Pro Taper和K锉预备新鲜健康离体牙,随机分为3组,A组:52.5 g/L次氯酸钠(Na Cl O)冲洗;B组:30 m L/L过氧化氢液和52.5 g/L Na Cl O交替冲洗;C组:170 g/L EDTA和52.5 g/L Na Cl O交替冲洗。D组:为培养的h DPSCs,作为空白对照组。牙髓干细胞分别接种于各组牙本质中,扫描电镜(SEM)和MTT法分别观察细胞的粘附和增殖情况,并检测ALP的活性和矿化基因的表达。结果:A、B、C组h DPSCs均生长粘附良好。其中C组SEM下细胞数量最多,表现出最强的增殖能力;且该组细胞显示出较高的碱性磷酸酶活性和矿化基因表达水平。结论:经标准根管预备EDTA处理的根管壁可促进h DPSCs的增殖和向成牙本质细胞分化。AIM：To compare the effects of dentin surfaces treated by different endodontic irrigation on the proliferation and differentiation of human dental pulp stem cell （ hDPSCs） . METHODS：The extracted healthy human teeth were cleaned and shaped using ProTaper and K-type file rotary instrumentation. The irrigation treatments investi-gated were 52. 5 g/L sodium hypochlorite （group A）, 30 mL/L hydrogen peroxide and 52. 5 g/L sodium hypochlorite （group B）, 170 g/L EDTA and 52. 5 g/L sodium hypochlorite （group C）. hDPSCs were seeded on the treated dentin slices with the density of 2 × 105/mL. Cell adhesion and proliferation on the dentin slices were observed by SEM and MTT method. Cell differentiation was evaluated by ALP activity assay and Real-Time PCR. RESULTS：hDPSCs ad-hered well on all dentin slices. The cells in group C showed more proliferation higher ALP level and mRNA expression of OC, DSPP and DMP-1 than those in group A and B. CONCLUSION：Dentin slices treated by EDTA-root canal preparation can promote proliferation and odontogenic differentiation of DPSCs.

关 键 词：牙本质 牙髓干细胞 组织工程 

分 类 号：R780.2[医药卫生—口腔医学]