可注射牙槽骨修复材料引导牙周组织再生的动物研究  

Influence of a novel injectable alvelor bone substitutes on regeneration of periodontal defects with experimental periodontitis in dogs

作　　者：张智星[1] 冯祥礼[2] 毛靖[1] 肖建中[3] 邱进俊[4] 刘承美[4] 

机构地区：[1]华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉430030 [2]湖北省中医院口腔科,湖北武汉430061 [3]华中科技大学材料科学与工程学院,湖北武汉430079 [4]华中科技大学化学学院,湖北武汉430079

出　　处：《临床口腔医学杂志》2015年第6期326-329,共4页Journal of Clinical Stomatology

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA03Z0443)

摘　　要：目的:建立实验动物牙周炎模型,评价含四环素的新型可注射牙槽骨修复材料用于引导牙周组织再生的实际效果。方法:选用15只雄性、健康的杂交犬,在双侧上颌尖牙的近中根面放置不锈钢网6周,建立实验动物牙周炎模型。在上颌尖牙近中牙槽骨处制作3壁骨内袋缺损,分别随机将含有1%、5%、10%四环素(TTC)的不饱和聚磷酸酯/β-磷酸三钙复合物(UPPE/β-TCP)直接注射入一侧的牙周骨缺损区(实验组),另一侧不植入任何材料(空白对照组)。术后16周处死动物,常规制备组织病理学样本,对实验组和空白对照组牙周组织的再生情况进行组织学和形态学评价,并对实验结果进行单因素方差分析和组间多重比较。结果:植入UPPE/β-TCP/TTC复合物的牙周骨缺损区内均可见新骨和新牙骨质形成等牙周组织再生现象,无明显的炎症反应,部分UPPE/β-TCP/TTC复合材料被新生骨组织所替代,且新生骨组织、新生牙骨质和新生牙周结缔组织附着宽度与空白对照组间的差异均有统计学意义(P<0.05)。结论:含TTC的新型可注射牙槽骨修复材料UPPE/β-TCP复合物可为牙周骨缺损区的愈合提供稳定的空间,并促进牙周组织的愈合,有望用于牙周组织再生治疗。Objective：To evaluate the influence of the injectable alveolar bone substitute on regeneration of peri-odontal defects with experimental periodontitis in dogs. Method：Experimental periodontitis was induced by placing stain-less-steel mesh on the mesial side of maxillary canines for 6 weeks in fifteen adult,healthy dogs. Subsequently,intrabony defects were resized so as to be standard,and UPPE/β-TCP composite containing 1 %,5 % and 10 % TTC were directly injected in the experimental bone defects. Non-grafted defects on the contralateral side served as controls. Sixteen weeks af-ter surgery,the animals were sacrificed and histologic specimens were prepared. Periodontal tissue healing was evaluated histologically and histometrically. Result：Healing of periodontal tissues,in terms of bone and cementum formation,was con-sistently observed in the UPPE/β-TCP/TTC composite-applied sites. UPPE/β-TCP/TTC composites were partly re-placed by new bone. New cementum and periodontal ligament-like tissue were observed between UPPE/β-TCP/TTC composites and the root surface. New bone,new cementum,and new connective tissue attachment and adhesion were signifi-cantly enhanced in the experimental sites. Conclusion：The UPPE/β-TCP/TTC composites containing TTC provides sta-ble wound healing and enhanced periodontal regeneration in periodontal defects in dogs with experimental periodontitis. The local application of injectable bioerodible UPPE/β-TCP/TTC composites appears to be promising in the context of peri-odontal treatment.

关 键 词：不饱和聚磷酸酯/β-磷酸三钙 四环素 牙周炎 牙周组织再生 

分 类 号：R781.4[医药卫生—口腔医学]