大鼠实验性牙周炎治疗前后血清中IL-18与sTREM-1的浓度变化  

Changes of Serum IL-18 and sTREM-1 Levels in Experimental Periodontitis in Rats Before and After Treatment

作　　者：石丽萍[1] 何艳艳[1] 王丽[1] 

机构地区：[1]哈尔滨医科大学附属口腔医院牙周病科,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2015年第6期591-593,共3页Journal of Oral Science Research

摘　　要：目的:观察大鼠实验性牙周炎基础治疗前后血清中IL-18与sTREM-1水平的变化,进而探讨IL-18与sTREM-1水平变化与牙周炎治疗效果间的关系。方法:选取2个月大的健康雄性Wistar大鼠30只,同等条件下饲养,随机分为3组,每组10只。正常对照组(A组)不做其他处理;牙周炎组(B组)与牙周炎治组(C组),将消毒尼龙丝线放置在双侧上颌第二磨牙牙颈部,同时配合牙周炎食谱诱导大鼠牙周炎模型。建模成功后C组去除丝线进行基础治疗,2周后对大鼠进行腹主动脉取血并处死,采用酶联免疫吸附法(ELISA)测定大鼠血清中IL-18和sTREM-1水平。结果:通过HE染色观察各组牙周组织学改变,牙周炎组(B组)大鼠血清中IL-18及sTREM-1水平高于正常对照组(A组)和牙周炎治疗组(C组)(P<0.05),且牙周炎治疗组大鼠血清中IL-18及sTREM-1水平高于对照组(P<0.05)。结论:牙周炎时IL-18、sTREM-1水平明显增高,且经基础治疗后明显降低。表明IL-18、sTREM-1水平可以为牙周炎的诊断和预测提供指导,为临床工作者提供准确有效的信息,同时也为临床治疗提供新的治疗靶点。Objective：To observe the changes of serum IL-18（interleukin18）and sTREM-1（striggering receptor1 expressedon myeloid cells,TREM-1）levels before and after the foundation treatment in rats with periodontitis and explore the relationship between serum IL-18,sTREM-1levels and response to treatment.Methods：30healthy 2-month-old male Wistar rats were randomly divided into three groups（n=10）.The rats with no other processing as the normal controls（Group A）.The second premolars of group B and group C were ligated with silk,fed with a periodontitis recipe.After the success of modeling,we removed the silk of C group and then gave basic treatment.After two weeks,abdominal aortic blood and rats were killed,the level of IL-18 and sTREM-1in serum were measured by ELISA.Results：In untreated periodontitis group the level of IL-18 and sTREM-1in serum were significantly higher than the control group and the treatment of periodontitis-based group（ P〈0.05）and the level of IL-18 and sTREM-1in serum of the treatment of periodontitis-based group was higher than the control group.Conclusion：The level of IL-18 and sTREM-1in serum significantly increased in periodontitis groups,and obviously reduced after basic treatment.This indicated that the IL-18 and sTREM-1levels could provide guidance for the diagnosis and prognosis of periodontitis,provide accurate and useful information for clinical workers,also could provide new therapeutic targets for clinical treatment.

关 键 词：大鼠 牙周炎 IL-18 STREM-1 

分 类 号：R781.42[医药卫生—口腔医学]