Numb 基因负性调节 CXCR4表达抑制膀胱癌细胞 BIU-87的增殖和迁移  

Numb inhibits proliferation and migration of human bladder cancer cell line BIU-87 by negative modu-lating CXCR4

作　　者：司马晋[1] 张保[1] 王保军[2] 于源滋[3] 艾青[2] 张旭[2] 

机构地区：[1]航天中心医院(北京大学航天临床医学院)泌尿外科,北京100049 [2]解放军总医院泌尿外科,北京100049 [3]山东省立医院消化科

出　　处：《国际肿瘤学杂志》2015年第7期492-495,共4页Journal of International Oncology

基　　金：国家自然科学基金（81100561）;航天科工集团医疗卫生科研项目（2014-LCYL-007）

摘　　要：目的：研究 Numb 基因对趋化因子受体4（CXCR4）表达的影响以及对膀胱癌细胞增殖和迁移的影响。方法实验分为3组：实验组（在膀胱癌细胞 BIU-87中转染 Numb-ORF 表达质粒）、阴性对照组（转染空载体）和空白对照组（未行转染处理）。使用荧光定量 PCR 和 Western blotting 检测各组细胞 Numb 和 CXCR4的表达。使用 MTS 法和 Transwell 小室法检测细胞增殖和迁移能力。结果实验组、阴性对照组和空白对照组中 Numb 基因表达水平分别为31.044±3.350、4.401±0.567和4.287±0.341，差异有统计学意义（F ＝183.418，P ＝0.000）；实验组、阴性对照组和空白对照组的 CXCR4表达水平分别为0.344±0.167、0.996±0.148和1.010±0.106，差异有统计学意义（ F ＝21.355，P ＝0.002）。细胞增殖检测中实验组、阴性对照组和空白对照组的48 h 吸光度值分别为0.615±0.057、0.987±0.063和0.957±0.066，差异有统计学意义（F ＝33.210，P ＝0.001）。迁移实验中，实验组、阴性对照组和空白对照组的穿膜细胞数分别为（164.667±19.858）个、（670.133±38.760）个和（667.533±27.610）个，差异有统计学意义（F ＝286.788，P ＝0.000）。结论 Numb 可能通过负性调节CXCR4表达抑制膀胱癌细胞的增殖和迁移。Objective To study the effect of Numb gene on CXCR4 expression and proliferation and migration of bladder cancer. Methods Human bladder cancer cells were divided into 3 groups：experimental group（transfected with Numb-ORF plasmid）,negative-control group（ transfected with blank vector）,and blank-control group（no DNA transfected in）. The expressions of Numb and CXCR4 were detected by real-time PCR and Western blotting respectively. Cell proliferation and migration were measured by MTS and Transwell assay respectively. Results Numb expressions in experimental group,negative-control group and blank-control group were 31. 044 ± 3. 350,4. 401 ± 0. 567 and 4. 287 ± 0. 341 respectively,with a statistical significance （F = 183. 418,P = 0. 000）. CXCR4 levels in experimental group,negative-control group and blank-control group were 0. 344 ± 0. 167,0. 996 ± 0. 148 and 1. 010 ± 0. 106 respectively,with a statistical significance （F = 21. 355,P = 0. 002）. In MTS assay,the absorb values in experimental group,negative-control group and blank-control group were 0. 615 ± 0. 057,0. 987 ± 0. 063 and 0. 957 ± 0. 066,with a statistical difference（F =33. 210,P = 0. 001）. In Transwell assay,the numbers of migratory cells in experimental group,negative-con-trol group and blank-control group were 164. 667 ± 19. 858,670. 133 ± 38. 760 and 667. 533 ± 27. 610,with a statistical significance（F = 286. 788,P = 0. 000）. Conclusion Overexpression of Numb can suppress the ability of proliferation and migration of the BIU-87 cells through down-regulation of CXCR4 expression in human＆amp;nbsp;bladder cancer.

关 键 词：膀胱肿瘤 受体 CXCR4 细胞增殖 细胞迁移测定 

分 类 号：R737.14[医药卫生—肿瘤]