HBsAg对脂多糖诱导树突状细胞分泌IL-6和IL-12的影响  

Effect of HBsAg on IL-6/IL-12 production of mononuclear-derived dendritic cells stimulated by lipopolysaccharide

作　　者：汤永志[1] 燕飞[1] 朱坚胜[1] 陈华忠[1] 朱敏[2] 肖明[3] 林希[1] 邵辉[1] 

机构地区：[1]温州医科大学附属台州医院感染科,台州317000 [2]温州医科大学附属台州医院公共科研平台,台州317000 [3]重庆医科大学病理教研室

出　　处：《浙江医学》2015年第11期974-976,共3页Zhejiang Medical Journal

基　　金：浙江省医药卫生科学研究基金计划项目(2010KYB125)

摘　　要：目的研究HBsAg对脂多糖诱导树突状细胞(DCs)分泌IL-6和IL-12的影响。方法采用重组人粒-单核集落刺激因子(rhGM—CSF)联合重组人白细胞介素4(rhIL-4)诱导单个核细胞得到未成熟树突状细胞(iDCs),分别加入HB—sAg1、2、5μg/ml,并设对照组,各组再加入脂多糖诱导为成熟DCs。采用流式细胞术鉴定各组iDCs的表型(CD11c、HLA—DR),ELISA法检测脂多糖诱导后培养上清液中IL-6和IL-12的水平。结果 iDCs的CD11C/HLA—DR双阳性率为(83.62±6.89)%,显著高于单纯培养组(20.57±11.19)%,差异有统计学意义(P<0.05)。HBsAg 1、2、5μg/ml组培养上清液中IL-6分别为(609.36±127.06)、(566.01±173.46)、(295.03±76.08)pg/ml,低于对照组(1356.97±181.78)pg/ml(均P<0.05);HBsAg2、5μg/ml组IL-12分别为(854.49±67.92)、(472.09±55.70)pg/ml,低于对照组(1248.78±112.09)pg/ml(均P<0.05);1μg/ml组IL-12(1 103.53±134.15)pg/ml,与对照组比较差异无统计学意义(P>0.05)。结论低浓度HBsAg可下调脂多糖诱导的DCs细胞因子分泌。Objective To investigate the effect of HBsAg on IL-6/IL-12 production of dendritic cells （DCs） stimulated by Lipopolysaccharide （LPS）. Methods Mononuclear cells were isolated and cultured in complete medium containing GM-CSF plus IL-4 to obtain immature DCs. Flow cytometry were performed to define the phenotypic characteristics of iDCs. Different con- centrations （1, 2 and 5wg/ml） of HBsAg were added in culture medium. The DCs were cultured for 24 h for maturation, followed by a 24h-differentiation culture with LPS （1μg/ml）. The inflammatory cytokines from cell supernatant were determined by en- zyme-linked immunosorbent assay （ELISA）. Results The expression of CD11c and HI_A-DR on immature DCs was （83.62± 6.89） %, compare to （20.57 ± 11.19） % in control group. IL-6 production was significantly decreased with the treatment of HBsAg （1, 2 and 5 μg/ml, P〈0.05）. IL-12 production demonstrates the similar results with the treatment of 2 and 5μg/ml HBsAg （P〈 0.05）, but no changes with the treatment of 1 w g/ml HBsAg （P 〉0.05）. Conclusion HBsAg may down-regulate the secretion of cytokines in DCs stimulated by LPS at lower dosage.

关 键 词：乙型肝炎表面抗原 树突状细胞 IL-6 IL-12 INTERLEUKIN-6 INTERLEUKIN-12 

分 类 号：R512.62[医药卫生—内科学]