降钙素基因相关肽对蛛网膜下腔出血模型大鼠脑血管痉挛的影响  

Effects of calcitonin gene related peptide in cisterna magna on cerebral vasospasm after experimental subarachnoid hemorrhage in rats

作　　者：吴庆建[1] 孙树印[1] 宋大庆[1] 张颜波[2] 闫承军[1] 孙保亮[2] 

机构地区：[1]济宁市第一人民医院急诊内科,济宁272011 [2]泰山医学院附属医院神经内科

出　　处：《中华行为医学与脑科学杂志》2015年第6期489-492,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（30770759）

摘　　要：目的 研究经枕大池应用降钙素基因相关肽（calcitonin gene related peptide,CGRP）对蛛网膜下腔出血（subarachnoid hemorrhage,SAH）大鼠模型脑血管痉挛的影响.方法 64只雄性Wistar大鼠随机分为4组：A组为正常对照组,B～D组在SAH模型建立后,经枕大池分别给予生理盐水、CGRP及腺病毒CGRP.全自动血液分析仪检测脑脊液白细胞、酶联免疫法检测CGRP活性、激光共聚焦显微镜观察循环内皮细胞、激光多普勒血流计检查顶叶皮层局部脑血流、数字减影血管造影检测基底动脉痉挛及血液气体分析仪分析动脉血气.结果 各组大鼠给药前及给药后白细胞数差异无统计学意义（P＞0.05）,动脉血气差异无统计学意义（P＞0.05）.脑脊液中CGRP浓度：给药48 h后,与对照组比较,B组（0.006±0.003）nmol/L,差异无统计学意义（P＞0.05）;C组（1.160±0.170） nmol/L,增加约200倍,差异有统计学意义（P＜0.05）;D组（2.071 ±0.412） nmol/L,增加近400倍,差异有统计学意义（P＜0.05）.外周血循环内皮细胞计数变化：给药后48 h,C组[（5.56±0.61）个/0.9 μL]较B组[（9.94±0.73）个/0.9 μL]减少（P＜0.01）,D组[（5.16±0.61）个/0.9 μL]较C组减少（P＜0.01）.顶叶皮层局部脑血流：给药后,与B组比较,C组和D组的脑血流升高,差异有统计学意义（均P＜0.01）.基底动脉直径：给药后12 h,D组大鼠[（1.000±0.025） mm]比B组[（0.670±0.028） mm]大13％ （P＜0.05）,比C组[（0.900±0.023）mm]大3％（P＞0.05）.结论 CGRP经枕大池给药有效改善SAH后的脑血管痉挛,且经枕大池注射腺病毒CGRP效果比CGRP更好。Objective To explore the effects of application calcitonin gene related peptide（CGRP） in cisterna magna on cerebral vasospasm after experimental subarachnoid hemorrhage （subarachnoid hemorrhage,SAH） in rat models.Methods 64 male Wistar rats were randomly divided into 4 groups.Group A was normal control group.After the subarachnoid hemorrhage models were established,group B,C,D were given normal saline,CGRP and adenovirus CGRP through cisterna magna respectively.White blood cells in cerebrospinal fluid were detected by automatic blood analyzer,CGRP activity was detected by enzyme linked immunosorbent assay,circulating endothelial cells were observed through laser scanning confocal microscope and parietal cortex regional cerebral blood flow were observed by laser doppler flowmeter.Basilar artery vasospasm and arterial blood gas analysis were detected by digital subtraction angiography and blood gas analyzer respectively.Results Before and after administration,there were no statistical differences in white blood cells and artery blood gas among the 4 groups （both P〉 0.05）.After administration 48 h,compared with group A,concentrations of CGRP in cerebrospinal fluid group B （0.006±0.002） did not increase （P〉0.05）,but increased 200 times in Group C （（1.160±0.170） nmol/L,P〈0.05）and nearly 400 times in group D （（2.071±0.412） nmol/L,P〈0.05）.Peripheral blood circulating endothelial cells count：after administration 48 h,group C（（5.56±0.61） ind/0.9 μL） was less than in group B（（9.94± 0.73） ind/0.9 μL）.Group D（（5.16±0.61） ind/0.9 μL） was less than group C（P〈0.01）.Regional cerebral blood flow：after administration,compared with group B,cerebral blood flow of group C and group D increased,and the differences were both statistically significant （P〈0.01）.Basilar artery diameter was detected after administration 12 h,group D （（1.000±0.025） mm） was 13% bigger than group B （（0.670±0.028）mm,P〈0.05）,3% bigger than group C �

关 键 词：降钙素基因相关肽 大鼠 蛛网膜下腔出血 脑血管痉挛 

分 类 号：R743.35[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]