微小RNA-203在骨肉瘤中的表达及其生物学意义  

Expression of microRNA-203 and its biological significance in osteosarcoma

作　　者：李振伟[1] 吴学建[1] 肖鹏[1] 朱旭[1] 

机构地区：[1]郑州大学第一附属医院骨科,450052

出　　处：《中华实验外科杂志》2015年第7期1566-1568,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察微小RNA （miRNA）-203在骨肉瘤中表达及其作用.方法 采用反转录-聚合酶链反应（RT-PCR）检测35例骨肉瘤患者癌及癌旁组织miRNA-203表达水平,同时检测骨肉瘤细胞株（MG-63、HOS、U2OS）与正常成骨细胞株hFOB1.19的miRNA-203表达.通过噻唑蓝比色法（MTT）与Transwell实验观察miRNA-203对MG-63增殖、侵袭的影响.结果 相对于癌旁组织与hFOB1.19细胞,miRNA-203在骨肉瘤癌组织与骨肉瘤细胞株表达显著下调（P＜0.01）.在MG-63细胞株过表达miRNA-203可显著抑制细胞增殖（P＜0.01）,48、72 h的抑制率分别为21.32％、30.20％,miRNA-203组侵袭细胞数[（50±8）个]显著低于空载体组的（131±5）个（P＜0.05）.结论 miRNA-203在骨肉瘤中表达明显降低.Objective To explore the expression and role of microRNA （miRNA）-203 in osteosarcoma.Methods Reverse transcriptase-polymerase chain reaction （RT-PCR） assays were used to detect the expression of miRNA-203 in human osteosarcoma and adjacent normal tissues.The expression of miRNA-203 in osteosarcoma cell lines （MG-63,HOS,U2OS） and normal osteoblast cell hFOB1.19 was also tested.Methyl thiazol tetrazolium （MTT） and Transwell assays were applied to observe the role of miRNA-203 in proliferation and invasion of MG-63.Results miRNA-203 was significantly down-regulated in human osteosarcoma tissues and MG-63 as compared with adjacent normal tissues and hFOB 1.19 （P ＜ 0.01）.Overexpression of miRNA-203 inhibited MG-63 proliferation （P ＜ 0.01）.At the time points of 48 and 72 h,the inhibition rate was 21.32％,and 30.20％ respectively.Morever,the invasion cells of miRNA-203 overepression （50 ± 8） were significantly less than those of control group （131 ± 5,P ＜ 0.05）.Conclusion miRNA-203 may play an important role in the osteosarcoma progression.

关 键 词：骨肉瘤 微小RNA-203 增殖 侵袭 

分 类 号：R739.7[医药卫生—肿瘤]