低频脉冲电磁场对激素性股骨头坏死骨组织过氧化物酶体增殖物激活受体-γ2及Runt相关转录因子2表达的影响  

Effects of low-frequency pulsed electromagnetic fields therapy on peroxisome proliferator-activated receptor-γ2 and Runt-related transcription factor 2 expression in steroid-induced avascular necrosis of femoral head in rats

作　　者：李建平[1] 陈森[1] 彭昊[1] 方洪松[1] 周建林[1] 

机构地区：[1]武汉大学人民医院骨关节外科,430060

出　　处：《中华实验外科杂志》2015年第7期1578-1581,共4页Chinese Journal of Experimental Surgery

摘　　要：目的 观察低频脉冲电磁场（LF-PEMF）对大鼠激素性股骨头坏死（SANFH）骨组织过氧化物酶体增殖物激活受体-γ2（PPAR-γ2）及Runt相关转录因子2（Runx2）表达的影响.方法 将42只Wistar大鼠随机分为3组：（1）模型组（S,n=16）,尾静脉注射10 μg/kg大肠杆菌内毒素;24 h后臀肌注射20 mg/kg甲基泼尼松龙,24h 1次,共3次.造模后不予磁场照射;（2）磁场治疗组（S＋P,n=16）,造模方法同前,4周后给予磁场照射（15 Hz,4.5 ms,1.2 mT）,4 h/d,共8周;（3）空白对照组（C,n=10）,不造模,不予磁场照射.磁场治疗8周后处死所有大鼠,取股骨头组织行苏木素-伊红（HE）染色,采用反转录酶-聚合酶链锁反应（RT-PCR）及Western blot检测骨组织内PPAR-γ2及Runx2的表达.结果 S组SANFH发病率（69％）及空骨陷窝率[（59.55±21.70）％]明显高于S＋P组[31％、（36.16±15.34）％,P＜0.05]及C组[0％、（8.79±2.42）％,P＜0.05];S组股骨头组织内PPAR-γ2 mRNA及蛋白的表达显著高于S＋P组及C组,差异有统计学意义（P＜0.05）;而Runx2mRNA及蛋白的表达则显著低于S＋P组及C组,差异有统计学意义（P＜0.05）.结论 LF-PEMF是一种无创、安全而有效的治疗早期SANFH的方法,可能机制是下调骨坏死骨组织内PPAR-γ2的表达,上调Runx2的表达,抑制脂肪生成,促进骨形成,降低骨坏死率.Objective This study was designed to study the effects of low-frequency pulsed electromagnetic fields （LF-PEMF） therapy on peroxisome proliferator-activated receptor-γ2 （PPAR-γ2） and Runt-related transcription factor 2 （Runx2） expression in steroid-induced avascular necrosis of femoral head （SANFH）.Methods Forty-two Wistar rats were divided into three groups.（1） Steroid group （S,n =16）,animal modeling was making by glucocorticoid and lipopolysaccharide,without exposure to LF-PEMF.（2） Steroid ＋ LF-PEMF group （S ＋ P,n =16）,four weeks after making modeling,all rats were treated with LF-PEMF （4.5 ms square pulse,repeated at 15 Hz,with a peak of 1.2 mT） for4 h/d for the next 8 weeks.（3）Control group （C,n =10）,all rats were treated without steroid use and exposure to LF-PEMF.After 8-week treatment,the histological changes,the osteonecrosis incidence,the empty osteocyte lacuna rate and the mRNA and protein expression of PPAR-γ2 and Runx2 were measured and analyzed.Results In Group S,the osteonecrosis incidence （69％） and empty osteocyte lacuna rate [（59.55 ± 21.70） ％] were both higher than which in group S ＋ P [31％,（36.16 ± 15.34） ％;P ＜ 0.05] and group C [0％,（8.79 ± 2.42）％;P ＜ 0.05].Furthermore,in group S,the expressions of PPAR-γ2 mRNA and protein are significantly higher than that in group S ＋ P and group C （P ＜ 0.05）,and the expressions of Runx2 mRNA and protein are significantly lower than that in group S ＋ P and group C （P ＜ 0.05）.Conclusion LF-PEMF is an effective and safe physiotherapy in the treatment of SANFH,and the possible underlying mechanisms include increasing the expressions of Runx2 and decreasing the expressions of PPAR-γ2 in femoral head tissues,and further promoting bone formation and inhibiting adipogenesis.

关 键 词：脉冲电磁场 股骨头缺血坏死 糖皮质激素 过氧化物酶体增殖物激活受体-γ2 Runt相关转录因子2 

分 类 号：R782[医药卫生—口腔医学]