MPTP致帕金森模型小鼠海马区NGF基因的表达研究  

Expression of hippocampus NGF in MPTP mouse model of Parkinson's disease

作　　者：曹丽[1] 夏艳秋[1] 刘鑫[1] 胥玲[1] 王湘娴 陆军[1,2] 郑元林[1,2] 

机构地区：[1]江苏师范大学生命科学学院,江苏徐州221116 [2]江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《中国药理学通报》2015年第7期957-961,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81400902;81271225;81171012;30950031);江苏高校优势学科建设工程资助项目(No PAPD);2012年度江苏高校青蓝工程(No12qld008);江苏省高校自然科学研究重大项目(No13KJA180001);江苏省高校自然科学基金(No 09KJB180009);江苏省重点实验室资助项目(No KLBMP1403);徐州师范大学自然科学基金(No 08XLR09;09XLY05;09XKXK02;10XL A05);国家级大学生创新创业训练计划项目(No 12ssjcx23)

摘　　要：目的检测帕金森(Parkinson’s disease,PD)模型小鼠海马区(hippocampus)神经生长因子(nerve growth factor,NGF)基因的表达。方法采用腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)建立PD小鼠模型;利用蛋白免疫印迹(Western blot)法和免疫荧光技术(immunofluorescence,IF)检测中脑酪氨酸羟化酶(tyrosine hydroxylase,TH)表达鉴定PD模型建立是否成功;采用足迹分析法和避暗法分别检测小鼠运动能力和认知能力;利用RT-PCR和原位杂交技术(in situ hybridization)检测小鼠海马区NGF基因的表达。结果与对照组相比,模型组的TH、NGF水平均降低,行为学指标偏离正常值,且差异有显著性。结论 NGF可能在PD认知障碍的病理学发生、发展过程中发挥重要作用。Aim To detect the expression of hippocam-pus NGF gene in the mouse model of Parkinson 's dis-ease. Methods By intraperitoneal injection of 1-meth-yl-4-phenyl-1,2, 3, 6-tetrahydropyridine ( MPTP), a mice model of Parkinson ’ s disease was established. The success of PD model was identified by detecting the expression of mesencephalic tyrosine hydroxylase ( TH) with Western blot and immunofluorescence. The movement and cognitive ability of mice were evaluated by foot print analysis and step-through passive avoid-ance test. The expression of NGF gene in hippocampus of mice was detected with RT-PCR and in situ hybrid-ization. Results Compared with the control group, the levels of TH and NGF were reduced in the experi-mental group and the behavioral indexes were deflected significantly. Conclusion NGF may play an impor-tant role in the occurrence and development of the PD cognitive disorder.

关 键 词：MPTP 帕金森 TH 海马区 NGF 认知能力 

分 类 号：R-332[医药卫生] R322.81