羟基红花黄色素A通过影响PCNA表达和MEK-ERK1/2信号通路抑制大鼠血管平滑肌细胞增殖  

Hydroxysafflor yellow A inhibits VSMCs proliferation via PCNA and MEK-ERK1/2

作　　者：赵京山[1,2] 方明星[3] 郭浅妤 李云峰[1] 徐丙元[1] 赖少鸿 张赏月 刘玉[1] 李爱英[1] 

机构地区：[1]河北中医学院基础医学院生物化学与生物学教研室,河北石家庄050200 [2]河北省心脑血管病中医药防治重点实验室,河北石家庄050200 [3]河北医科大学第三医院,河北石家庄050052 [4]河北医科大学,河北石家庄050017

出　　处：《中国药理学通报》2015年第7期984-988,共5页Chinese Pharmacological Bulletin

基　　金：河北省科技支撑计划(No 13277739D);河北省卫生厅重点科技研究计划资助项目(No 20100043)

摘　　要：目的探讨羟基红花黄色素A(HYSA)影响增殖细胞核抗原(PCNA)表达和MEK-ERK1/2信号通路抑制血管平滑肌细胞(VSMCs)增殖的分子机制。方法采用贴块法培养大鼠血管平滑肌细胞,用血小板源生长因子(PDGF)诱导其增殖,采用MTT法检测HYSA对平滑肌细胞增殖的抑制作用;采用Western blot和免疫组织化学方法检测HYSA对PCNA表达及对MEK-ERK1/2信号通路的影响。结果HYSA浓度依赖性地抑制PDGF诱导的VSMC增殖,降低PCNA的表达,阻断PDGF受体的激活和MEK/ERK信号通路的活化,但不影响JNK和p38MAPK的活性。结论 HYSA通过降低PCNA表达和阻断MEK-ERK1/2信号通路抑制大鼠血管平滑肌细胞增殖。Abstrac:Aim To study the effect of hydroxysafflor yellow A ( HYSA ) on the proliferation of vascular smooth muscle cells ( VSMCs) and the related molecu-lar mechanism. Methods The inhibitory effects of hydroxysafflor yellow A on VSMC proliferation was de-tected using cell culture, MTT assay, Western blot and immunohistochemical staining. Results The results showed that HYSA inhibited cell proliferation induced by PDGF in a dose-dependent (5,10,20,40 μmol· L-1 ) manner, reduced proliferating cell nuclear anti-gen ( PCNA ) expression and blocked PDGFR-MEK-ERK1/2 signaling pathway activated by PDGF in VSMCs. Conclusion HYSA inhibits VSMCs prolifer-ation via reducing the expression of PCNA and blocking signal transduction of MEK-ERK1/2 in VSMCs.

关 键 词：羟基红花黄色素A 血管平滑肌细胞 增殖细胞核抗原 丝裂原活化蛋白激酶 细胞外信号调节激酶 细胞增殖 

分 类 号：R-332[医药卫生] R284.1