HPLC-DPPH评价剑叶龙血树中抗氧化活性成分及构效关系  

Evaluation on anti-oxidant capacity of Dracaena cochinchinensis by HPLC-DPPH and analysis on structure-activity relationship

作　　者：周艳林[1,2,3] 闵建国[2] 邹准[2] 刘华钢[3] 邹节明[2] 陈士林[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]桂林三金药业股份有限公司,广西桂林541004 [3]广西医科大学药学院,广西南宁530021

出　　处：《中草药》2015年第12期1797-1799,共3页Chinese Traditional and Herbal Drugs

基　　金：广西科技攻关项目(0630002-2M;10124008-15);广西医药产业人才小高地资助项目(0802)

摘　　要：目的采用HPLC-DPPH法评价剑叶龙血树主要成分的抗氧化活性并分析其构效关系。方法采用HPLC法测定剑叶龙血树中自藜芦醇、龙血素A、龙血素B、紫檀芪、7,4′-二羟基黄酮,色谱条件:PhenomenexluraC_(18)(250mmn×4.6mmn,5μm)色谱柱,甲醇-乙腈-0.2%磷酸溶液梯度洗脱,体积流量1.0 mL/min,柱温35℃,检测波长306 nm。通过对比剑叶龙血树药材溶液加1,1二苯基-2-三硝基苯肼自由基(DPPH)反应前后的各化合物色谱峰面积衰减情况,以各化合物峰面积比值计算清除率,评价各化合物抗氧化活性。结果二苯乙烯类、二氢查耳酮类成分为剑叶龙血树中主要抗氧化活性成分;抗氧化能力强弱依次为紫檀芪(清除率73.19%)>白藜芦醇(清除率71.10%)>龙血素B(清除率39.01%)>龙血素A(清除率12.14%)。从构效关系上分析,二苯乙烯类化合物,当羟基甲基化后,其抗氧化能力并未减弱;二氢查耳酮类化合物,苯环上甲氧基取代成对称结构有助于发挥其抗氧化能力;而仅有C7,4′位羟基取代的黄酮类化合物未显示抗氧化活性。结论建立的HPLC-DPPH评价方法能量化表达剑叶龙血树中主要抗氧化成分,为筛选药效指标成分及建立全面谱效相关质量标准奠定基础。Objective To evaluate the antioxidant capacity of the main components from Dracaena cochinchinensis by HPLC-DPPH and provide a foundation for ＂spectrum-effect＂ quality control standard.Methods The determination was developed on Phenomenex lura C18 column（250 mm×4.6 mm,5μm） with gradient elution of methanol-acetonitril-0.2%H3PO4 and the detective wavelength was set at 306 nm.The column temperature was 35℃.Results The DPPH free radical scavenging abilities of these five antioxidants were as follows：pterstilbeneresveratrolluoreirin Bluoreirin A7,4＇-dihydroxyflavone.The analysis on the structures of the five compounds showed that the hydroxymethylation didn＇t decrease the antioxidant activities of stilbenes.The symmetry of B ring of dihydrochalcone has the significant effect on the antioxidant activities.However,7,4＇-dihydroxyflavone didn＇t show the related activity in D.cochinchinensis.Conclusion HPLC-DPPH cannot only be used for screening the components with antioxidant potency but also for the purpose of quality evaluation of D.cochinchinensis,and the method proves to be selective,simple,and reproducible.

关 键 词：剑叶龙血树 抗氧化活性 构效关系 HPLC-DPPH 白藜芦醇 龙血素A 龙血素B 紫檀芪 7 4′-二羟基黄酮 

分 类 号：R285.5[医药卫生—中药学]