几株非模式白腐菌降解能力的研究  

A Study on the Degradation Capacity of Several Non-model White-rot Fungi

作　　者：吴雪君[1] 崔宝凯[1] 

机构地区：[1]北京林业大学微生物研究所,北京100083

出　　处：《生物技术通报》2015年第6期151-156,共6页Biotechnology Bulletin

摘　　要：利用愈创木酚选择培养基,从11种白腐菌株中定性筛选出3株白腐菌,分别是毡毛栓孔菌Trametes velutina Dai10149、鍺栓孔菌T.ochracea Cui 6888、绒毛栓孔菌T.pubescens Cui 7571。重量法绘制它们的生长曲线,同时测定其蛋白质含量及胞外酶活。通过比较发现,绒毛栓孔菌T.pubescens Cui 7571的生长速度快,且具有较强及稳定的酶活分泌能力;对针叶植物、阔叶植物及单子叶草本的木质纤维素均表现出较好的降解效果。Based on testing of guaiacol selective medium, 3 isolates from 11 white-rot fungi were selected, and they areTrametes velutina Dai 10149,T. ochraceaCui 6888 and T. pubescens Cui 7571. Then growth curve of the 3 isolates based on weight method were plotted, and the changes of protein content and two extracellular enzyme activities were monitored. The results showed thatT. pubescens Cui 7571 had excellent ability of growth, and stable and outstanding ability of secreting cellulase and laccase. It can significantly decompose the lignocelluloses in conifer, hardwood and monocotyledon plants.

关 键 词：白腐菌 生长曲线 酶活 组分含量 降解能力 

分 类 号：X172[环境科学与工程—环境科学]