乳腺癌前哨淋巴结分子病理检测技术的验证:RT-LAMP与GeneSearch技术的对比研究  

Verification of a molecular pathology detection method of sentinel lymph node in breast cancer patients: a comparison between RT-LAMP and Gene Search

作　　者：林镪[1] 刘毅[1] 梁峰[1] 刘荫华[2] 段学宁[2] 王翔[3] 欧阳涛 蒋宏传[5] 李波[6] 杨德起[7] 郝晓鹏[1] 尉承泽[1] 

机构地区：[1]军事医学科学院附属医院,北京100071 [2]北京大学第一医院,北京100034 [3]中国医学科学院肿瘤医院,北京100021 [4]北京大学肿瘤医院,北京100142 [5]首都医科大学附属朝阳医院,北京100020 [6]北京医院,北京100730 [7]北京大学人民医院,北京100044

出　　处：《军事医学》2015年第5期373-376,共4页Military Medical Sciences

摘　　要：目的验证课题组前期建立的乳腺癌前哨淋巴结(SLN)术中快速分子病理检测技术逆转录环介导等温扩增技术(reverse-transcription loop-mediated isothermal amplification,RT-LAMP)的可靠性。方法分别应用RTLAMP法与经过美国FDA批准的Gene Search技术检测乳腺癌患者的SLN,比较两种方法检测结果的一致性。结果收集北京市7家三甲医院134例乳腺癌SLN样本,以Gene Search技术为金标准,RT-LAMP方法的敏感性为96.2%(25/26),特异性为96.3%(104/108),两种方法的一致率为96.3%(129/134),统计学一致性分析Kappa=0.8857,P<0.001。结论课题组前期建立的RT-LAMP方法与Gene Search技术相比有较高的一致性,具有快速、便捷、低成本等特点,在乳腺癌SLN术中分子病理检测方面具有良好的应用前景。Objective To verify the reliability of our previously established reverse-transcription loop-mediated isother-mal amplification （ RT-LAMP） method for the detection of sentinel lymph nodes metastasis in breast cancer patients .Meth-ods Sentinel lymph nodes of breast cancer patients were analyzed by RT-LAMP and FDA-approved GeneSearch methods respectively, and the consistency of the two methods was assessed with a kappa concordance test.Results One hundred and thirty-four cases of sentinel lymph node samples were collected from seven hospitals in Beijing .Using the GeneSearch assay as the gold standard, the sensitivity, specificity and consistentcy of RT-LAMP were 96.2%（25/26）,96.3%（104/108） and 96.3%（129/134）, respectively.Statistical analysis showed that the two methods were consistent （Kappa=0.8857, P＆lt;0.001）.Conclusion RT-LAMP is highly consistent with GeneSearch ,efficient,simple and inexpensive, promising a good prospect of application to intra-operative detection of sentinel lymph nodes metastasis for breast cancer patients.

关 键 词：乳腺癌 前哨淋巴结 RT-LAMP技术 GeneSearch技术 

分 类 号：R737.9[医药卫生—肿瘤]