大鼠脑胶质瘤细胞培养上清液的收集及其乳酸含量变化和意义  

The collection of extracellular fluid of C6cell culture and the significance of lactic acid content content

作　　者：高丹丹[1] 刘君星[2] 辛华[1] 李雅江[1] 王琳[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003 [2]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2015年第3期44-45,47,共3页Heilongjiang Medicine and Pharmacy

基　　金：佳木斯大学研究生科技创新面上项目;编号:LM2014-023;黑龙江省卫生厅项目;编号:2011-401

摘　　要：目的:验证胶质瘤细胞(C6)可能的一种乳酸快速排泌机制。方法:C6按6组不同浓度(0.5×105;0.75×105;1×105;1.25×105;1.5×105;2×105)接种,24h收集上清液,检测乳酸,乳酸脱氢酶含量,以海马神经元HT22作为对照。结果:接种浓度为1×105个/L组细胞24h密度适中处于指数增生期,乳酸和乳酸脱氢酶含量高于其他组(P<0.01)(除1.25×105个/L浓度组)。C6细胞培养上清液中乳酸含量明显高于HT22细胞培养上清液(P<0.01)。结论:胶质瘤细胞可能有一种快速排泌机制,使得其代谢产生的大量乳酸在细胞外液中堆积。Objective： To validate the quick exhaust urinary mechanism of lactic acid glioma cell.Methods：C6cells were inoculated according to different concentrations in 6 groups（0.5 × 10^5; 0.75 × 10^5; 1 × 10^5; 1.25 ×10^5; 1.5 × 10^5; 2 × 10^5）.The supernatant was collected after 24 hours.The contents of lactic acid and lactate dehydrogenase were determinated with hippocampal neurons HT22 as control.Results： The number of cells is moderate and cells grow at an exponential rate in the group of 1 × 10^5/ L inoculation concentration after 24 h.Lactic acid and lactate dehydrogenase were higher than those in other groups（P 0.01）（except for 1.25 × 10^5 concentration group）.Lactic acid content of C6 cell culture supernatant was significantly higher than that in HT22cell（P 0.01）.Conclusion： Glioma cells may have a rapid excretion mechanism.The accumulation of large amounts of lactic acid makes the metabolized in the extracellular fluid.

关 键 词：脑胶质瘤 细胞培养 乳酸 乳酸脱氢酶 

分 类 号：R446.6[医药卫生—诊断学]