福寿螺和田螺的纤维素酶反应体系对不同pH和温度的响应  

Responses of cellulase reaction systems of Pomacea canaliculata and Cipangopaludina chinensis to different pH and temperature

作　　者：罗明珠[1,2] 章家恩[2] 胡九龙[3] 赵本良[2] 邓智心[2] 

机构地区：[1]华南农业大学农学院,广东广州510642 [2]华南农业大学热带亚热带生态研究所,广东广州510642 [3]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2015年第4期55-58,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金(U1131006;30770403;30900187);广东省科技计划项目(2011B020309009);广东省高等学校高层次人才项目(粤教师函【2010】79号);广东省引进国(境)外高层次人才智力项目(粤外专[2010]51号)

摘　　要：【目的】探讨福寿螺Pomacea canaliculata Lamarck和本地中国圆田螺Cipangopaludina chinensis Gray纤维素酶活性反应体系对不同p H和温度响应的差异.【方法】采用酶学分析法（羧甲基纤维素法）测定不同反应温度和p H对福寿螺和田螺肝脏与胃纤维素酶活性的影响.【结果和结论】福寿螺肝脏和胃纤维素酶的活性在不同p H和温度条件下均显著高于田螺,其中,不同p H（3.6~7.2）条件下福寿螺肝脏和胃纤维素酶活性分别是田螺的1.51~3.14和1.30~1.90倍;不同温度（30~60℃）条件下福寿螺肝脏和胃纤维素酶活性分别是田螺的1.16~1.56和1.32~1.77倍.在p H3.6~7.2、温度30~60℃的范围内,福寿螺和田螺肝脏、胃纤维素酶活性都呈先上升后降低的变化趋势,不同温度条件下福寿螺纤维素酶活性的变化幅度大于田螺,但2种螺纤维素酶活性随p H的变化差异不显著.福寿螺肝脏和胃纤维素酶适宜的p H范围分别为5.4~6.6和4.8~6.6,适宜的温度范围均为40~55℃;田螺肝脏和胃纤维素酶适宜的p H范围分别为5.4~6.0和4.8~6.6,适宜的温度范围均为40~50℃.福寿螺对外界环境的适应性更强.【Objective】To investigate the effects of p H and temperature on cellulose enzyme activities of Pomacea canaliculata Lamarck and Cipangopaludina chinensis Gray. 【Method】The enzymatic analysis method（ Carboxymethyl cellulose method） was used to research the influences of different reaction temperatures and p H values on cellulose enzyme activities in liver and stomach of P. canaliculata and C.chinensis. 【Result and conclusion】The cellulose enzyme activities in liver and stomach of P. canaliculata were significantly higher than those of C. chinensis under different p H and temperature. The cellulose enzyme activities in liver and stomach of P. canaliculata at different p H values（ 3. 6- 7. 2） were 1. 51-3. 14 and 1. 30- 1. 90 times higher than those of C. chinensis,respectively. At different temperatures（ 30- 60 ℃）,the cellulose enzyme activities were 1. 16-1. 56 and 1. 32- 1. 77 times higher than thoseof C. chinensis,respectively. At the settled p H（ 3. 6- 7. 2） and temperature（ 30- 60 ℃） conditions,the curves of cellulase activities were all rising followed by reducing. At different temperatures,the variation ranges of cellulase activities in liver and stomach of P. canaliculatas were wider than those of C. chinensis,while there was no significant differcence between P. canaliculata and C. chinensis at different p H values. The optimum p H range for cellulose enzyme activities in liver and stomach of P. canaliculata were 5. 4- 6. 6 and 4. 8- 6. 6,respectively; the optimum temperature range was from 40 ℃ to 55℃. Meanwhile, The optimum p H ranges for cellulose enzyme activities in liver and stomach of C. chinensis were 5. 4- 6. 0 and 4. 8- 6. 6,respectively; the optimum temperature ranged from 40 ℃ to50 ℃. P. canaliculata has a better digestive ability and is more adaptive to the environment which may attribute to the easier invasion mechanism of P. canaliculata.

关 键 词：福寿螺 田螺 纤维素酶 反应体系 PH 温度 胃 肝脏 

分 类 号：S954.3[农业科学—水产养殖]