咪达唑仑与丙泊酚对局灶缺血再灌注大鼠脑水肿影响的对照研究  

Comparative study on influences of midazolam and propofol on rat brain edema induced by ischemia/reperfusion injury

作　　者：徐进步[1] 施青山[1] 许鹏程[2] 王露[2] 马艳艳[1] 汪允珍[1] 

机构地区：[1]江苏省连云港市第二人民医院重症医学科,222000 [2]徐州医学院江苏省麻醉学重点实验室,江苏徐州221004

出　　处：《检验医学与临床》2015年第13期1834-1836,共3页Laboratory Medicine and Clinic

基　　金：蚌埠医学院科研课题计划(byky13123)

摘　　要：目的对比咪达唑仑与丙泊酚对脑局灶缺血再灌注后脑水肿状态的影响。方法雄性SD大鼠72只随机分为四组:假手术组、脑缺血再灌注组、丙泊酚处理组、咪达唑仑处理组。采用大脑中动脉线栓法建立局灶性脑缺血模型,缺血2h再灌注72h行神经功能缺损评分、断头取脑行脑梗死体积和脑含水量测量以及脑组织水通道蛋白4(AQP4)表达的检测。结果与缺血再灌注组相比,咪达唑仑与丙泊酚均能明显降低脑梗死体积,减轻脑水肿,降低AQP4的表达,两组差异无统计学意义(P>0.05)。结论咪达唑仑具有与丙泊酚同等地减轻脑局灶缺血再灌注后水肿的作用,适合作为该类疾病状态下的镇静药。Objective To compare the influences of midazolam and propofol on brain edema induced by ischemia/reperfusion（I/R）injury.Methods 72 male Sprague-Dawley（SD）rats were randomized into four groups：sham operation group undergoing sham operation;ischemia/reperfusion group undergoing the thread embolism of the left middle cerebral artery occlusion（MCAO）for 2hand then removement of thread;propofol treatment group,which accepted propofol treatment on the basis of ischemia/reperfusion for 72h;midazolam treatment group,which accepted midazolam treatment on the basis of ischemia/reperfusion for 72 h.After 72 hof reperfusion the rats were assessed and assigned the neurological deficit scores,then decapitated to evaluate the cerebral infarction volumes,brain water contents and the expression of AQP4.Results In the comparison with the I/R group,both the midazolam treatment group and the propofol treatment group could improve the neurological score,ameliorate brain edema,and down-regulate the expression of AQP4,the differences had no statistical significance（P〉0.05）.Conclusion Midazolam is equally potent as propofol in ameliorating brain edema induced by I/R injury,and is a suitable sedative for such condition.

关 键 词：咪达唑仑 丙泊酚 缺血再灌注损伤 脑水肿 水通道蛋白 

分 类 号：R743[医药卫生—神经病学与精神病学]