高强度聚焦超声阻滞腰交感神经节对后肢动脉闭塞动物模型脊髓P物质和外周血β-内啡肽的影响  

Effect of lumbar sympathetic ganglion blocking with high intensity focused ultrasound on substancep of spinal cord and β-endorphine of peripheral blood in hind limb arterial occlusion animal model

作　　者：傅洪[1] 魏安宁[2] 李发琪[3] 王智彪[3] 

机构地区：[1]重庆市中医院麻醉科,400021 [2]重庆医科大学附属第二医院麻醉科,重庆400010 [3]重庆医科大学超声生物医学工程研究所,重庆400016

出　　处：《检验医学与临床》2015年第13期1852-1854,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨高强度聚焦超声（HIFU）行腰交感神经节阻滞对周围动脉闭塞动物血浆β-内啡肽（β-EP）及脊髓P物质（SP）的影响。方法 26只家犬按随机数字表法随机分为三组：HIFU治疗组（A组,n=12）,非治疗组（B组,n=12）,正常对照组（C组,n=2）。A、B两组建立后肢缺血动物模型,A组术后2周采用HIFU阻滞第2-6腰交感神经节,B组不予处理。A、B两组每次随机选2只犬在治疗后不同时间点采用放射免疫法检测血浆β-EP,取腰段脊髓行免疫组织化学染色检测SP变化;C组直接检测。结果 （1）A、B两组在造模后2周血浆β-EP水平均低于C组（P〈0.01）,治疗后A组不同时间点β-EP水平高于B组（P〈0.05）。（2）脊髓SP阳性表达主要分布在脊髓背角Ⅰ、Ⅱ层。A、B两组在造模后2周脊髓腰段SP的OD值明显高于C组（P〈0.01）,HIFU干预后A组脊髓腰段SP的OD值低于B组（P〈0.05）。（3）犬周围动脉闭塞缺血后血浆β-EP与脊髓SP的变化呈负相关。结论HIFU阻滞腰交感神经节治疗慢性周围动脉闭塞缺血动物模型,可有效升高血浆β-EP水平和降低脊髓SP水平,提高痛觉阈值,减轻疼痛。Objective To observe the effect of lumbar sympathetic ganglion blocking with high intensity focused ultrasound（HIFU）on substance P（SP）of spinal cord andβ-endorphine（β-EP）in blood plasma in arterial occlusion animal model.Methods 26 canis familiaris were randomly assigned to 3groups：HIFU therapy group（group A,n=12）,non-therapy group（group B,n=12）and normao control group（group C,n=2）.In group A and B,the arteria cruralis was liberated and all branches（including arteria profunda femoris）were cutted,then a helical tinsel was inserted into the arteria.In group A,the 2nd- 6th ganglion sympatheticums were blocked with HIFU after postoperative 2weeks.In every time point after the therapy,2dogs were randomly selected from groups A and B every time forβ-EP examination,and then the spinal cord SP was detected,dogs in group C were directly detected.Results（1）Comparison with the group C,β-EP in the groups A and B was significantly decreased（P〈0.01）,β-EP in the group A was higher than that in the group B after therapy（P〈0.05）.（2）In the groups A and B,SP was located in the I,Ⅱlayers of dorsal horn in spinal cord,postoperative average optical density（OD）of SP was significantly higher than that in the group C（P〈0.01）;the OD in the group A was less than that in the group B after therapy（P〈0.05）.（3）In the model dog of arteria occlusion,blood plasmaβ-EP was negative correlated with the SP in the spinal cord.Conclusion Lumbar sympathetic ganglion blocking for hind limb arterial occlusion animal model by HIFU can increase blood plasmaβ-EP and decrease SP of spinal cord,and elevate the pain threshold value and then relieve pain.

关 键 词：后肢动脉闭塞 腰交感神经节阻滞 P物质 Β-内啡肽 疼痛 

分 类 号：R741[医药卫生—神经病学与精神病学]