磁性碳纳米管载附5-FU对胃癌淋巴结转移模型抑制作用研究  

INHIBITORY EFFECT OF MAGNETIC CARBON NANOTUBES LOADED WITH 5-FU VEHICLE IN LYMPH NODE METASTASIS MODEL OF GASTRIC CANCER

作　　者：金彦召[1] 曹家庆[1] 胡小云[1] 毛盛勋[1] 沈威[1] 贾惊宇[1] 李正宇[1] 辛林[1] 

机构地区：[1]南昌大学第二附属医院,江西南昌330006

出　　处：《现代医院》2015年第7期4-6,共3页Modern Hospitals

基　　金：江西省教育厅青年基金资助项目(编号:GJJ13040)

摘　　要：目的观察磁性多壁碳纳米管在胃癌淋巴结化疗中的作用。方法 MTT法检测mMWNTs(3 mg/ml)、mMWNTs-5-FU(3 mg/ml-12 mg/ml)、5-FU(12 mg/ml)对胃癌SGC7901细胞的抑制作用。将mMWNTs(3 mg/ml)、mMWNTs-5-FU(3 mg/ml-12 mg/ml)、5-FU(12 mg/ml)分别注入SD大鼠足垫皮下及胃癌淋巴结转移裸鼠体内。结果体外mMWNTs(3 mg/ml)、mMWNTs-5-FU(3 mg/ml-12 mg/ml)、5-FU(12 mg/ml),经稀释(10-10000)倍后,相同5-FU浓度时两者对体外SGC7901细胞的抑制作用无显著性差异,说明主要药物成分是5-FU。结论体内研究提示mMWNTs-5-FU能有效聚集在淋巴结,淋巴结浓集效果明显优于5-FU(p<0.05),并能缓慢控制释放,且不良反应小;mMWNTs-5-FU化疗后胃癌淋巴结转移灶癌细胞有明显凋亡现象,说明mMWNTs-5-FU对胃癌SGC7901细胞淋巴结转移有明显抑制作用。Objective To observe the effect of magnetic multi -walled carbon nanotubes in chemotherapy of lymph node of gastric cancer. Methods MTT assay was adopted to measure the inhibitory effects of different concentrations （ diluted 10 - 10000 times） of mMWNTs（3 mg/ml） , mMWNTs-5 -FU（3 mg/ml～ 12 mg/ml） , 5 -FU（ 12 mg/ml） on the growth of SGC7901 cells. mMWNTs（3 mg/ml） , mMWNTs-5 -FU（3 mg/ml ～ 12 mg/ml） and 5 -FU （12 mg/ml） were injected into subcutaneous footpad of SD rat and lymph node metastasis of gastric cancer in nude mice. Results After dilution （ diluted 10 - 10000 times） of mMWNTs （3 mg/ml） , mMWNTs-5 -FU（3 mg/ml -12 mg/ml） and 5 -FU （12 mg/ml） in vitro, the inhibitory effect had no significant difference among the same concentration of 5 - FU on SGC7901 cells, which indicated that the main ingredient was 5 - FU. Conclusion In vivo studies indicate that mMWNTs -5 -FU can effectively gather in the lymph nodes, whose lymph-node concentration effect is obviously better than that of 5 -FU （p 〈 0. 05 ）, with a slow controlled release and fewer adverse reactions. The obvious cell apoptosis of lymph node metastasis cells after the mMWNTs - 5 - FU chemotherapy, indicates that mMWNTs - 5 - FU has a significant inhibitory effect on SGC7901 cells of lymph node metastasis of gastric cancer.

关 键 词：磁性多壁碳纳米管 淋巴靶向化疗 胃癌 淋巴结转移 

分 类 号：R735.2[医药卫生—肿瘤]