山莓叶抑制家兔离体肠管运动活性物的分离纯化及活性研究  

Isolation,purification and activity of active components from Rubus corchorifolius leaves and their inhibition effects on movement of isolated rabbit intestinal

作　　者：周丽萍[1] 陈雪香[2] 刘晓娟[2] 常云佩 肖林平[1] 钟晨晨[1] 曹庸[2] 

机构地区：[1]中国广州分析测试中心/广东省分析测试技术公共实验室,广东广州510070 [2]华南农业大学食品学院,广东广州510642

出　　处：《广东农业科学》2015年第11期108-112,共5页Guangdong Agricultural Sciences

基　　金：广东省科技计划农业攻关项目(2012A020602038);2011年高等学校博士学科点专项科研基金(20114404120022)

摘　　要：为明确山莓叶中抗腹泻的药效成分,利用溶剂萃取、常压柱层析、中压柱层析对山莓叶中的活性成分进行分离纯化,采用家兔离体肠实验检测抗腹泻活性,并对活性单体进行结构鉴定。结果表明:山莓叶中抑制离体肠效果好的化合物是对羟基肉桂酸乙酯,当其浓度为20 mg/m L时,离体肠张力增量的变化率达到70.0%,强于阿托品的抑制效果。In order to ensure the anti-diarrhea components from Rubus corchorifolius leaves, the active components from R. corchorifolius leaves were isolated and purified using solvent extraction, column chromatography and middle pressure column chromatography, and their anti-diarrhea activities were determined through the movement of isolated intestinal of rabbit, meanwhile, the structure of active monomer was identified. The results showed that the component which had anti-diarrhea activity on isolated intestinal was P-hydroxy ethyl einnamate in R. corchorifolius leaves, when the concentration was 20 mg/mL, the change rate of tension increment of isolated intestinal reached 70.0%, and its inhibition effect was better than that of atropine.

关 键 词：山莓叶 离体肠 分离纯化 对羟基肉桂酸乙酯 

分 类 号：S853.71[农业科学—临床兽医学]