深低温冻存对牙周膜干细胞膜片增殖和分化能力的影响  

Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheet

作　　者：魏福兰[1,2] 马晓妮[2,3] 张凡[1,2] 

机构地区：[1]山东大学口腔医院正畸科,山东济南250012 [2]山东省口腔生物医学重点实验室,山东济南250012 [3]山东大学口腔医院种植科,山东济南250012

出　　处：《口腔生物医学》2015年第2期87-89,共3页Oral Biomedicine

基　　金：国家自然科学基金(81470709);山东省自然科学基金(ZR2011HQ017)

摘　　要：目的:研究深低温冻存对牙周膜干细胞膜片增殖和分化能力的影响。方法:将牙周膜干细胞膜片用90%胎牛血清+10%DMSO冻存液冻存3月后复苏,采用甲基噻唑基四唑(MTT)法、von Kossa染色和油红O染色法检测冻存对增殖和分化的影响。结果:冻存牙周膜干细胞膜片与新鲜牙周膜干细胞膜片的增殖率和成骨、成脂分化能力没有明显差异。结论:牙周膜干细胞膜片经深低温保存后可继续保持冻存前原有的增殖能力和多向分化能力。Objective：To investigate the effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cells （PDLSCs）sheet.Methods：We compared cryopreserved PDLSCs sheet with freshly PDLSCs sheet in terms of cell proliferation rate and multilineage differentiation potential by MTT,von Kossa staining and Oil Red staining.Results：Cryopreserved PDLSCs sheet showed a similar cell proliferation rate and multilineage differentiation potential as fresh PDLSCs sheet.Conclusions：This study demon-strates that cryopreservation does not affect the proliferation and differentiation of PDLSCs sheet,supporting the feasibility of PDLSCs sheet cryopreservation in nitrogen.

关 键 词：牙周膜干细胞膜片 冻存 细胞增殖 细胞分化 

分 类 号：R780.2[医药卫生—口腔医学]