两种fimA型牙龈卟啉单胞菌对人脐动脉平滑肌细胞增殖的影响  

Effects of two fim A variants of Porphyromonas gingivalis on the function of human umbilical artery smooth muscle cells

作　　者：贾惠杰 李文军[1] 葛颂[1] 

机构地区：[1]遵义医学院附属口腔医院牙周科,贵州遵义563003

出　　处：《口腔生物医学》2015年第2期95-99,共5页Oral Biomedicine

基　　金：国家自然科学基金项目(81260168;30760271);贵州省优秀青年科技人才培养对象专项资金[黔科合人字(2011)32号]

摘　　要：目的:探究两种fim A型牙龈卟啉单胞菌对人脐动脉平滑肌细胞增殖的影响。方法:采用组织块贴壁法培养人脐动脉平滑肌细胞。在厌氧条件下培养Ⅰfim A、Ⅳfim A型牙龈卟啉单胞菌,并与人脐动脉平滑肌细胞共同培养;在共培养2、8、24和48 h时,采用CCK-8法检测细胞生长状况。结果:Ⅰfim A型牙龈卟啉单胞菌刺激人脐动脉平滑肌细胞后8 h,促增殖作用明显,但是24和48 h平滑肌细胞增殖受到抑制(P<0.05);Ⅳfim A型牙龈卟啉单胞菌对人脐动脉平滑肌细胞没有明显促细胞增殖或细胞毒性作用。结论:Ⅰfim A型和Ⅳfim A型牙龈卟啉单胞菌对人脐动脉平滑肌细胞的作用存在差异,Ⅰfim A型牙龈卟啉单胞菌有明显的促平滑肌细胞增殖和细胞毒性作用。Objective：To explore effects of viability on human umbilical artery smooth muscle cells affected by two different fimA-genetypes of Porphyromonas gingivalis.Methods：The tissue-block attached method was used for primary culture of human umbilical ar-tery smooth muscle cells.Porphyromonas gingivalis was cultured in the anaerobic conditions.Human umbilical artery smooth muscle cells were invaded by fimA genotype Ⅰ and Ⅳstrains respectively for 2,8,24 and 48 h.The CCK-8 assay was applied in testing viabili-ty of smooth muscle cells.Results：After fimA typeⅠstrains were cultured with vascular smooth muscle cells,smooth muscle cells were significantly increasing at 8 h,but obviously,cytotoxicity appeared at 24 h and 48 h （P 〈0.05）.Cells invaded by fimA type Ⅳ Porphy-romonas gingivalis did not have any significant change as compared with that of control group.Conclusions：There are diversities be-tween the two different strains.FimA type Ⅰ strain plays a role in the processes of facilitating proliferation and cytotoxicity of vascular smooth muscle cells.

关 键 词：牙龈卟啉单胞菌 人脐动脉平滑肌细胞 fimA 型 CCK-8 动脉粥样硬化 

分 类 号：R780.2[医药卫生—口腔医学]