氧化应激反应在颞下颌关节内粘连形成中作用的研究  

The study of oxidative stress correlated with intracapsular adhesion formation in temporomandibular joint

作　　者：朱耀旻[1] 郑苍尚[1] 梁志刚[1] 邓永强[1] 胡欣欣[1] 周晓敏[1] 

机构地区：[1]深圳大学第一附属医院(深圳市第二人民医院)口腔颌面外科,广东省518035

出　　处：《中华临床医师杂志（电子版）》2015年第11期127-129,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：深圳市科技计划项目(医疗卫生类201302084)

摘　　要：目的通过兔颞下颌关节内氧化应激-粘连模型的建立和氧化应激反应的检测,阐明氧化应激在此过程中的作用机制并应用关节镜技术证实粘连的形成。方法取20只6月龄家兔进行分组实验,动物的左侧颞下颌关节局麻下于关节上腔内注入不同浓度的H2O2为实验侧,右侧关节作为空白对照侧。根据干预的不同梯度浓度分为4个浓度组(每组5只):0.5 mmol/L,2 mmol/L,5 mmol/L,10 mmol/L,连续注射7 d,每天2 ml进入关节腔后头颅绷带限制下颌活动正常进食。各组在注射后的第30天,应用颞下颌关节镜下检查各组家兔实验侧和对照侧关节内滑膜组织的变化和关节内粘连的形成情况,获得的致颞下颌关节内粘连的有效浓度的H2O2组动物和时间点,应用氧化应激检测试剂盒,酶联免疫吸附(ELISA)法检测实验侧和对照侧丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHd G,被公认为DNA氧化损伤和细胞氧化应激的指示剂)的变化。结果 4个浓度组中5 mmol/L,10 mmol/L组的动物,左侧关节腔内在第30天通过关节镜证实,获得了关节内粘连,右侧均无粘连的出现。抽取关节内滑液2 ml,12 000 r/min离心5 min,取上清100μl,ELISA试剂盒测定氧化应激检测,2,5,10 mmol/L组较空白对照组明显升高,差异有统计学意义(P<0.05)。结论成功建立氧化应激-关节内粘连的动物模型,证明5 mmol/L以上浓度的活性氧自由基H2O2可通过氧化应激途径使颞下颌关节发生病理性变化,形成关节腔内的粘连。Objective Direct exposure of ceUs to reactive oxygen species can induce ＇4 intracapsular adhesion （IA） in temporomandibular joint by establishing oxidative stress-intracapsular adhesion animal＇models with infusing ROS into articular cavity of rabbit TMJ. Methods The 20 rabbits were divided into four groups, oxidative stress was created with different gradient concentration of H202.（0.5 mmol/L, 2 mmol/L, 5 rrtmol/L, 10 mmol/L） into articular cavity of the left side of rabbit TMJs. And the right side of the joints was as blank control sides. 30 days after the injection, in the joints, arthroscopy And i xa^n~mofluorescence methods were used to examine the intra-articular synovial tissue and the situation of intracapsular adhesion change in different density of reactive oxygen species level to find perfect density to induce i^tracapsular adhesion. Results In the left side of rabbit TMJs of 5 mmol/L and 10 mmol/L groups, arthroscopy proofed ROS can form intracapsular adhesion through the oxidative stress way. There were no IA in all the right sides. ELISA methods examine of ROS in the synovial fluid, the 2 retool/L, 5 mmol/L and 10 mmol/L experimental groups compared to blank group was statistically significant. Conclusion We established oxidative stress-intraeapsular adhesion animal models of rabbit TMJ. Arthroscopy was used to proof over 5 mmol/L concentration of H202 can form intracapsular adhesion through the oxidative stress way.

关 键 词：颞下颌关节 关节镜 氧化应激反应 关节内粘连 

分 类 号：R782.6[医药卫生—口腔医学]