653份乳腺癌组织标本的ER-α36表达结果分析  

Analysis of ER-α36 expression in 653 cases of breast cancer tissues

作　　者：晋佳路[1] 朱仁书[1] 马全祥[2] 王云龙 

机构地区：[1]鹤壁职业技术学院,河南鹤壁458030 [2]新乡医学院,河南新乡450003 [3]河南省生物工程技术研究中心,河南郑州450001

出　　处：《国际检验医学杂志》2015年第13期1851-1853,共3页International Journal of Laboratory Medicine

摘　　要：目的研究雌激素受体α36亚型(ER-α36)在乳腺癌组织的表达状态及其与乳腺癌发生、发展及临床预后的关系。方法选取653份乳腺癌组织标本,用实时荧光定量聚合酶链反应(RT-PCR)技术和免疫组化技术检测组织中ER-α36、雌激素受体α66亚型(ER-α66)、孕激素受体(PR)和人表皮生长因子受体-2(Her-2)的表达,分析ER-α36的表达与上述指标及临床病理特征之间的关系。结果 ER-α36的总体表达率为40%;在Her-2阳性组织中的表达率为63%,明显高于阴性组的44%(P<0.05);在ER-α66/PR/Her-2三阴性组织中的表达率为66%,明显高于非三阴性组的35%(P<0.05);在ER-α66阳性和阴性标本中及在PR阳性和阴性标本中的表达率差异无统计学意义(P>0.05);在Ⅲ+Ⅳ期乳腺癌组织中的阳性表达率(54%)明显高于Ⅰ+Ⅱ期的28%(P<0.05);在淋巴结转移阳性乳腺癌组织中的表达率(55%)明显高于无转移组织的23%(P<0.05)。结论 ER-α36可能在乳腺癌的发生、发展及淋巴结转移过程中具有重要作用,并与Her-2的表达、乳腺癌分期及淋巴结转移有关,有望成为新的肿瘤标记物及临床诊治的新靶点。Objective To study the expression status of estrogen receptor-alpha36（ER-α36）in breast cancer tissue and its rela-tionships with the occurrence,development and clinical prognosis of breast cancer.Methods 653 cases of breast cancer tissues were selected in this study.The real-time reverse transcription polymerase chain reaction（RT-PCR）and immunohistochemistry were used to detect the expression of ER-α36,estrogen receptor-alpha66 （ER-α66）,progesterone receptor（PR）and human epidermal growth factor receptor-2（Her-2）.The relationships between the expression of ER-α36,ER-α66,PR and Her-2 and the pathological charac-ter were analyzed.Results The expression rate of ER-α36 in all cases was 40%.The expression rate of ER-α36 in Her-2 positive tissues（63%）was significantly higher than that in the Her-2 negative group（44%,P 〈0.05）.The expression rate of ER-α36 in ER-α66/PR/Her-2 negative tissues（66%）was significantly higher than that in the non-three-negative group（35%,P 〈0.05）.The differences of ER-α36 expression rate between ER-α66 positive samples and negative samples or between PR positive and negative samples showed no statistical significance（P 〉 0.05）.The expression rate of ER-α36 in stage Ⅲ＋Ⅳ breast cancer tissues（54%） was significantly higher than that in stage Ⅰ＋Ⅱ breast cancer tissues（28%,P 〈0.05）.The expression rate of ER-α36 in breast cancer tissues with lymph node metastasis （55%）was significantly higher than that in breast cancer tissues without lymph node me-tastasis （23%,P 〈0.05）.Conclusion The results indicate that ER-α36 may play a very important role in the occurrence,develop-ment and lymph node metastasis of breast cancer,and be associated with the expression of Her-2,breast cancer staging and lymph node metastasis.ER-α36 is expected to become a new tumor marker and clinical diagnosis and treatment target.

关 键 词：乳腺癌 雌激素受体α36亚型 雌激素受体α66亚型 孕激素受体 人表皮生长因子受体-2 

分 类 号：R737.9[医药卫生—肿瘤]