联合检测Rta-IgG、EBNA1-IgA和VCA-IgA抗体对中山地区鼻咽癌的诊断价值研究  

Diagnostic value of combined detection of Rta-IgG,EBNA1-IgA and VCA-IgA antibodies in nasopharyngeal carcinoma in Zhongshan area

作　　者：杜满兴[1] 王伟佳[1] 

机构地区：[1]中山市人民医院检验医学中心,广东中山528403

出　　处：《国际检验医学杂志》2015年第13期1856-1857,1860,共3页International Journal of Laboratory Medicine

摘　　要：目的评价联合检测EB病毒Rta蛋白抗体IgG(Rta-IgG)、核抗原1抗体IgA(EBNA1-IgA)和病毒衣壳抗原抗体IgA(VCA-IgA)对鼻咽癌(NPC)的临床诊断价值。方法收集416例经病理诊断确诊并未经治疗的NPC患者(NPC组)和688例体检健康者(对照组)的血清标本,均采用酶联免疫吸附试验检测血清Rta-IgG、VCA-IgA和EBNA1-IgA抗体。以临床病理诊断为金标准,分析各血清检测指标的准确度、灵敏度及特异度,并根据Rta-IgG、VCA-IgA和EBNA1-IgA抗体检测结果绘制受试者工作特征(ROC)曲线。结果 NPC组患者血清Rta-IgG、VCA-IgA和EBNA1-IgA抗体水平均高于对照组,差异均有统计学意义(P<0.05)。NPC组和对照组3种抗体均阳性者分别占56.25%、0.00%。Rta-IgG、VCA-IgA和EBNA1-IgA抗体的ROC曲线下面积分别为0.798、0.889和0.895,灵敏度分别为85.37%、87.56%和88.34%,特异度分别为93.45%、96.13%和96.37%。三者联合检测的灵敏度和特异度分别为96.48%、98.98%。结论联合检测Rta-IgG、VCA-IgA和EBNA1-IgA抗体能够明显提高临床对NPC患者的早期筛查与诊断效能,具有重要的临床应用价值。Objective To evaluate the diagnostic value of combined detection of IgG antibody against to Epstein-Barr virus （EBV）Rta protein（Rta-IgG）,IgA antibody against the EBV nuclear antigen 1（EBNA1-IgA）and IgA antibody against EBV viral capsid antigen （VCA-IgA）in nasopharyngeal carcinoma.Methods The serum samples of 41 6 patients with pathologically con-firmed nasopharyngeal carcinoma and 688 cases of healthy individuals were collected.Serum levels of Rta-IgG,VCA-IgA and EB-NA1-IgA antibodies were detected by using enzyme-linked immunosorbent assay.Taking the pathological diagnosis as gold stand-ard,the accuracy,sensitivity and specificity of the three serum indicators were analysed.And the receiver operating characteristic （ROC）curve were drawn according to the detecting results of Rta-IgG,EBNA1-IgA and VCA-IgA.Results The levels of Rta-IgG,EBNA1-IgA and VCA-IgA in the NPC group were significantly higher than those in the control group,the differences were statistically significant（P 〈0.05）.The NPC patients with three kinds of positive antibodies were positive accounted for 56.25%, while no in the combined examination of NPC group and the healthy control group appeared in 0%.The areas under the ROC curves of Rta-IgG,VCA-IgA and EBNA1-IgA were 0.798,0.889 and 0.895,respectively.The sensitivity of Rta-IgG,VCA-IgA and EB-NA1-IgA were 85.37%,87.56% and 88.34%,respectively;and the specificity were 93.45%,96.13% and 96.37%,respectively. The sensitivity and specificity of combined detection of these three antibodies were 96.48% and 98.98%,respectively.Conclusion The combined detection of Rta-IgG,VCA-IgA and EBNA1-IgA antibodies could significantly improve the efficacy of early screening and diagnosis in NPC patients,which has important clinical application value.

关 键 词：鼻咽癌 EB病毒 抗体 

分 类 号：R739.63[医药卫生—肿瘤]